Degradation of azo dye Mordant Yellow 10 in a sequential anaerobic and bioaugmented aerobic bioreactor

N. C G Tan, A. Borger, P. Slenders, A. Svitelskaya, G. Lettinga, James A Field

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Complete biodegradation of azo dyes requires an anaerobic and aerobic step, in the anaerobic step sulfonated azo dyes (SADs) are reduced, yielding (sulfonated) aromatic amines ((S)AAs) which can be degraded aerobically. The complete biodegradation of the SAD Mordant Yellow 10 (MY10) was studied in a sequential anaerobic and aerobic bioreactor. Anaerobically, MY10 was reductively cleaved and the resulting aromatic amines, 5-aminosalicylic acid (5-ASA) and sulfanilic acid (SA), were both recovered in high stoichiometric yields. One of the AAs, 5-ASA, was readily degraded under aerobic conditions. However, SA was not degraded aerobically in the continuous experiment because no SA-degrading bacterial activity was present in the system. Therefore, a SA-degrading enrichment culture derived from Rhine sediment was used as an inoculum source. This enrichment culture was bioaugmented into the aerobic reactor by increasing the hydraulic retention time (HRT), thus enabling SA-degrading activity to develop and maintain in the aerobic reactor. After decreasing the HRT, the SA-degrading activity remained in the bioreactor and the stoichiometric recovery of sulfate (a SA biodegradation product) indicated the mineralization of SA after bioaugmentation. Batch experiments with aerobic reactor sludge confirmed the biodegradation of SA and 5-ASA. The sequential anaerobic and aerobic bioreactor was able to completely remove the sulfonated azo dye MY10 at a maximum loading rate of 210 mg MY10 (l(reactor) d)-1 after the appropriate microorganisms for aerobic degradation of SA were bioaugmented into the aerobic bioreactor.

Original languageEnglish (US)
Pages (from-to)337-344
Number of pages8
JournalWater Science and Technology
Volume42
Issue number5-6
StatePublished - 2000
Externally publishedYes

Fingerprint

Azo dyes
Bioreactors
bioreactor
dye
Degradation
degradation
Acids
acid
Biodegradation
biodegradation
Amines
Hydraulics
hydraulics
oxic conditions
Microorganisms
microbial activity
Sediments

Keywords

  • 5-aminosalicylic acid
  • Azo dye reduction
  • Bioaugmentation
  • Mordant Yellow 10
  • Sequential anaerobic aerobic
  • Sulfanilic acid

ASJC Scopus subject areas

  • Water Science and Technology

Cite this

Tan, N. C. G., Borger, A., Slenders, P., Svitelskaya, A., Lettinga, G., & Field, J. A. (2000). Degradation of azo dye Mordant Yellow 10 in a sequential anaerobic and bioaugmented aerobic bioreactor. Water Science and Technology, 42(5-6), 337-344.

Degradation of azo dye Mordant Yellow 10 in a sequential anaerobic and bioaugmented aerobic bioreactor. / Tan, N. C G; Borger, A.; Slenders, P.; Svitelskaya, A.; Lettinga, G.; Field, James A.

In: Water Science and Technology, Vol. 42, No. 5-6, 2000, p. 337-344.

Research output: Contribution to journalArticle

Tan, NCG, Borger, A, Slenders, P, Svitelskaya, A, Lettinga, G & Field, JA 2000, 'Degradation of azo dye Mordant Yellow 10 in a sequential anaerobic and bioaugmented aerobic bioreactor', Water Science and Technology, vol. 42, no. 5-6, pp. 337-344.
Tan, N. C G ; Borger, A. ; Slenders, P. ; Svitelskaya, A. ; Lettinga, G. ; Field, James A. / Degradation of azo dye Mordant Yellow 10 in a sequential anaerobic and bioaugmented aerobic bioreactor. In: Water Science and Technology. 2000 ; Vol. 42, No. 5-6. pp. 337-344.
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