Demonstration of a neurokinin A receptor subtype in transfected fibroblasts

Alden K. Henderson, Henry I. Yamamura, Carlo A. Maggi, Stephen H. Buck, Paul L.M. Van Giersbergen, William R. Roeske

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

In competitive radioligand binding assays, the NK2 receptor antagonists [Tyr5,D-Trp6,8,9,Arg10]NKA(4-10) (MEN 10207) and [Tyr5,D-Trp6,8,9,Arg10]NKA(3-10) (MEN 10208) had high and low affinity, respectively, in bovine stomach membranes and SKLKB82#3 cells, a murine fibroblast cell line transfected with a cDNA encoding for the bovine NK2 receptor. These antagonists also had different affinities when inhibiting neurokinin A-induced polyphosphoinositide hydrolysis in SKLKB82#3 murine fibroblasts. Thus, the de novo protein expressed by the SKLKB82#3 murine fibroblasts may represent a distinct NK2 receptor subtype.

Original languageEnglish (US)
Pages (from-to)175-178
Number of pages4
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Volume225
Issue number2
DOIs
StatePublished - Feb 13 1992

Keywords

  • MEN 10207
  • MEN 10208
  • NK receptor subtype
  • Neurokinin A (NKA)

ASJC Scopus subject areas

  • Pharmacology

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