Demonstration of infectious taura syndrome virus in the feces of seagulls collected during an epizootic in Texas

J. R. Garza, K. W. Hasson, B. T. Poulos, R. M. Redman, B. L. White, Donald V Lightner

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Laughing gulls Larus atricilla were observed feeding on diseased shrimp Penaeus vannamei during an epizootic of the Taura syndrome virus (TSV) at a south Texas farm in May 1995. Fresh fecal samples were collected from a gull roosting site on a pond levee where gulls that fed on diseased shrimp had roosted overnight. The feces samples were tested for the presence of TSV by injection bioassay. A cell-free clarified homogenate of the fecal material was injected into indicator P. vannamei, and the shrimp were observed for 9 d. Cumulative mortalities of the two replicate groups of indicator shrimp were 45% and 70%. Moribund indicator shrimp collected and preserved for histological examination exhibited lesions pathognomonic for acute-phase infections by TSV. The presence of TSV in the induced lesions was confirmed with in situ hybridization with cDNA probes specific for a portion of the TSV genome. The presence of infectious TSV in the gulls' feces supports the hypothesis that these birds are a probable transport vector of the virus within and among nearby shrimp farms.

Original languageEnglish (US)
Pages (from-to)156-159
Number of pages4
JournalJournal of Aquatic Animal Health
Volume9
Issue number2
StatePublished - Jun 1997

Fingerprint

Taura syndrome virus
Laridae
Feces
feces
virus
shrimp
Charadriiformes
Viruses
Litopenaeus vannamei
lesions (animal)
lesion
farms
farm
Penaeidae
Larus
roosting
levee
in situ hybridization
Biological Assay
bioassays

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary(all)

Cite this

Demonstration of infectious taura syndrome virus in the feces of seagulls collected during an epizootic in Texas. / Garza, J. R.; Hasson, K. W.; Poulos, B. T.; Redman, R. M.; White, B. L.; Lightner, Donald V.

In: Journal of Aquatic Animal Health, Vol. 9, No. 2, 06.1997, p. 156-159.

Research output: Contribution to journalArticle

Garza, J. R. ; Hasson, K. W. ; Poulos, B. T. ; Redman, R. M. ; White, B. L. ; Lightner, Donald V. / Demonstration of infectious taura syndrome virus in the feces of seagulls collected during an epizootic in Texas. In: Journal of Aquatic Animal Health. 1997 ; Vol. 9, No. 2. pp. 156-159.
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