Dendritic cells transduced with HIV Nef express normal levels of HLA-A and HLA-B class I molecules

Lorraine A. Cramer, Jeffrey A Frelinger

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

HIV Nef protein is important for viral pathogenesis and disease progression. Nef downregulates CD4 and major histocompatibility antigens on the surface of HIV-infected T cells. HIV also infects dendritic cells. We wanted to determine if Nef had a similar function in professional antigen-presenting cells, where downregulation of Class I could have important effects on the initiation of HIV specific cytolytic T cell responses. We infected human dendritic cells with adenovirus expressing Nef. In contrast to T cells and Hela cells, HLA-A and HLA-B molecules are not downregulated nor are other class I molecules increased. We show that, in dendritic cells, HIV Nef has little effect on CD4 or Class I expression.

Original languageEnglish (US)
Pages (from-to)417-425
Number of pages9
JournalJournal of Acquired Immune Deficiency Syndromes
Volume27
Issue number5
Publication statusPublished - Aug 15 2001
Externally publishedYes

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Keywords

  • Adenovirus
  • Dendritic cell
  • HIV-Nef

ASJC Scopus subject areas

  • Virology
  • Immunology

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