Depletion of intracellular calcium stores facilitates the influx of extracellular calcium in platelet derived growth factor stimulated A172 glioblastoma cells

György Vereb, János Szöllösi, László Mátyus, Margit Balázs, William C. Hyun, Burt G. Feuerstein

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Calcium signaling in non-excitable cells is the consequence of calcium release from intracellular stores, at times followed by entry of extracellular calcium through the plasma membrane. To study whether entry of calcium depends upon the level of saturation of intracellular stores, we measured calcium channel opening in the plasma membrane of single confluent A172 glioblastoma cells stimulated with platelet derived growth factor (PDGF) and/or bradykinin (BK). We monitored the entry of extracellular calcium by measuring manganese quenching of Indo-1 fluorescence. PDGF raised intracellular calcium concentration ([Ca2+]i) after a dose-dependent delay (tdel) and then opened calcium channels after a dose-independent delay (tch). At higher doses (>3 nM), BK increased [Ca2+]i after a tdel ∼0 s, and tch decreased inversely with both dose and peak [Ca2+]i. Experiments with thapsigargin (TG), BK, and PDGF indicated that BK and PDGF share intracellular Ca2+ pools that are sensitive to TG. When these stores were depleted by treatment with BK and intracellular BAPTA, tdel did not change, but tch fell to almost 0 s in PDGF stimulated cells, indicating that depletion of calcium stores affects calcium channel opening in the plasma membrane. Our data support the capacitative model for calcium channel opening and the steady-state model describing quantal Ca2+ release from intracellular stores.

Original languageEnglish (US)
Pages (from-to)64-73
Number of pages10
JournalCytometry
Volume24
Issue number1
DOIs
StatePublished - May 1 1996
Externally publishedYes

    Fingerprint

Keywords

  • A172 glioblastoma
  • Bradykinin
  • Calcium signaling
  • Capacitative calcium entry
  • Image cytometry
  • Indo-1
  • PDGF
  • Thapsigargin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

Cite this