Derivatization and high-performance liquid chromatographic analysis of pentaazapentacosane pentahydrochloride

Susan Heimbecher, Yung Chi Lee, S. Esmail Tabibi, Samuel H. Yalkowsky

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A rapid high-performance liquid chromatographic method for determination of the dansyl derivative of pentaazapentacosane (PAPC) pentahydrochloride has been developed. The chromatographic system uses a reversed-phase C8 column, a mobile phase of acetic acid buffer and acetonitrile and UV detection. The dansylation conditions were optimized with a pH of 11.0 and a 20-fold dansyl chloride excess. The yield of dansyl PAPC increased 10-fold as the reaction pH was changed from 9.5 to 10.5. Under derivatization conditions of pH 8.5-11.0 and 1-30-fold excess dansyl chloride only perdansyl PAPC was found.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Volume691
Issue number1
DOIs
StatePublished - Mar 28 1997

Keywords

  • Dansylation
  • Pentaazapentacosane pentahydrochloride
  • Polyamines

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Derivatization and high-performance liquid chromatographic analysis of pentaazapentacosane pentahydrochloride'. Together they form a unique fingerprint.

  • Cite this