(Des-Histidine1) (Nε{lunate}-phenylthiocarbamoyllysine12)-glucagon: Effects on glycogenolysis in perfused rat liver

B. A. Khan, Marvin D. Bregman, C. A. Nugent, Victor J. Hruby, K. Brendel

Research output: Research - peer-reviewArticle

  • 13 Citations

Abstract

(Des-Histidine1) (Nε{lunate}-phenylthiocarbamoyllysine12)-glucagon, synthesized by the one-step Edman degradation procedure is a competitive inhibitor of glucagon action in the rat liver plasma membrane adenylate cyclase system. However, in the perfused rat liver, the compound did not inhibit glucagon stimulated glycogenolysis even when used at a concentration 100-fold in excess of native glucagon. Instead, it showed a weak potency, but full agonist activity, stimulating liver glycogenolysis to 100% of the level obtained by glucagon. These results are discussed in terms of the possible mechanism(s) of glucagon action.

LanguageEnglish (US)
Pages729-736
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume93
Issue number3
DOIs
StatePublished - Apr 14 1980

Fingerprint

Glycogenolysis
Glucagon
Histidine
Liver
Rats
Adenylyl Cyclases
Cell Membrane
Cell membranes
Degradation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

(Des-Histidine1) (Nε{lunate}-phenylthiocarbamoyllysine12)-glucagon : Effects on glycogenolysis in perfused rat liver. / Khan, B. A.; Bregman, Marvin D.; Nugent, C. A.; Hruby, Victor J.; Brendel, K.

In: Biochemical and Biophysical Research Communications, Vol. 93, No. 3, 14.04.1980, p. 729-736.

Research output: Research - peer-reviewArticle

@article{7281fc0f5827450daeb6d8c471adabd4,
title = "(Des-Histidine1) (Nε{lunate}-phenylthiocarbamoyllysine12)-glucagon: Effects on glycogenolysis in perfused rat liver",
abstract = "(Des-Histidine1) (Nε{lunate}-phenylthiocarbamoyllysine12)-glucagon, synthesized by the one-step Edman degradation procedure is a competitive inhibitor of glucagon action in the rat liver plasma membrane adenylate cyclase system. However, in the perfused rat liver, the compound did not inhibit glucagon stimulated glycogenolysis even when used at a concentration 100-fold in excess of native glucagon. Instead, it showed a weak potency, but full agonist activity, stimulating liver glycogenolysis to 100% of the level obtained by glucagon. These results are discussed in terms of the possible mechanism(s) of glucagon action.",
author = "Khan, {B. A.} and Bregman, {Marvin D.} and Nugent, {C. A.} and Hruby, {Victor J.} and K. Brendel",
year = "1980",
month = "4",
doi = "10.1016/0006-291X(80)91138-9",
volume = "93",
pages = "729--736",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - (Des-Histidine1) (Nε{lunate}-phenylthiocarbamoyllysine12)-glucagon

T2 - Biochemical and Biophysical Research Communications

AU - Khan,B. A.

AU - Bregman,Marvin D.

AU - Nugent,C. A.

AU - Hruby,Victor J.

AU - Brendel,K.

PY - 1980/4/14

Y1 - 1980/4/14

N2 - (Des-Histidine1) (Nε{lunate}-phenylthiocarbamoyllysine12)-glucagon, synthesized by the one-step Edman degradation procedure is a competitive inhibitor of glucagon action in the rat liver plasma membrane adenylate cyclase system. However, in the perfused rat liver, the compound did not inhibit glucagon stimulated glycogenolysis even when used at a concentration 100-fold in excess of native glucagon. Instead, it showed a weak potency, but full agonist activity, stimulating liver glycogenolysis to 100% of the level obtained by glucagon. These results are discussed in terms of the possible mechanism(s) of glucagon action.

AB - (Des-Histidine1) (Nε{lunate}-phenylthiocarbamoyllysine12)-glucagon, synthesized by the one-step Edman degradation procedure is a competitive inhibitor of glucagon action in the rat liver plasma membrane adenylate cyclase system. However, in the perfused rat liver, the compound did not inhibit glucagon stimulated glycogenolysis even when used at a concentration 100-fold in excess of native glucagon. Instead, it showed a weak potency, but full agonist activity, stimulating liver glycogenolysis to 100% of the level obtained by glucagon. These results are discussed in terms of the possible mechanism(s) of glucagon action.

UR - http://www.scopus.com/inward/record.url?scp=0018947495&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018947495&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(80)91138-9

DO - 10.1016/0006-291X(80)91138-9

M3 - Article

VL - 93

SP - 729

EP - 736

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -