Design and performance evaluation of reflection confocal microscopy using acousto-optical deflector and slit detector

Seung Woo Lee, Dong Kyun Kang, Hongki Yoo, Dae Gab Gweon, Suk Won Lee, Kwang Soo Kim

Research output: Contribution to journalConference article

1 Scopus citations

Abstract

We describe the design and the implementation of reflection confocal scanning microscopy (CSM) using an acoustooptical deflector (AOD) for the fast horizontal scan and a galvanometer mirror (GM) for the slow vertical scan. In the beam scanning system it is important to maintain the lateral and the axial performance during scanning operation. We propose a simple method to design a scanning system using the finite ray tracing and the diffraction theory. We define a cost function which contains the effect of aberrations on the performance of microscopy. We construct the designed system and evaluate its performance. The OSLO simulation shows that the performances of CSM are not changed with deflection angle. So we conclude that the beam scanning system is properly designed. In addition, we propose an image formation method and show images obtained with the system.

Original languageEnglish (US)
Article number43
Pages (from-to)235-241
Number of pages7
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5
Issue number13
DOIs
StatePublished - Dec 1 2004
Externally publishedYes
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI - San Jose, CA, United States
Duration: Jan 27 2004Jan 29 2004

Keywords

  • Acousto-Optical Deflector
  • Beam scanning system
  • Confocal microscopy
  • Finite ray tracing
  • Image formation
  • Relay optics
  • Slit detector

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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