Design of a multi-spectral channel for in-vivo confocal microscopy

Houssine Makhlouf, Anthony A. Tanbakuchi, Andrew R Rouse, Arthur F Gmitro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

We present a modified multi-spectral configuration of a slit-scanning confocal microendoscope that provides higher spectral resolution in a fully automated interface. Tissue fluorescence signal is directed through a dispersive element that spreads the spectral information across the CCD camera mapping spectral information perpendicular to the confocal slit. The dispersive element may be chosen to meet the specific requirements defined by the user. Our current system uses a BK7 prism with a 45° wedge angle and a 20mm diameter clear aperture. The prism is shifted from the optical axis allowing automated switching from grayscale (beam on-axis) to multi-spectral (beam off-axis) imaging by tilting a computer controlled mirror. The system records over a spectral range of 450nm to 750nm. The minimum resolvable wavelength difference varies from 2.1nm to 8.3nm over the spectral range. The lateral and axial resolution of the system is approximately 3μm by 30μm, respectively, and is the same for both grayscale and multi-spectral imaging modes. Multi-spectral imaging results from ex-vivo tissues are presented.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6432
DOIs
StatePublished - 2007
EventEndoscopic Microscopy II - San Jose, CA, United States
Duration: Jan 21 2007Jan 23 2007

Other

OtherEndoscopic Microscopy II
CountryUnited States
CitySan Jose, CA
Period1/21/071/23/07

Fingerprint

Confocal microscopy
Prisms
Imaging techniques
Tissue
Spectral resolution
CCD cameras
Mirrors
Fluorescence
Scanning
Wavelength

Keywords

  • Confocal
  • Dispersion
  • Microscopy
  • Prism
  • Spectral

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Makhlouf, H., Tanbakuchi, A. A., Rouse, A. R., & Gmitro, A. F. (2007). Design of a multi-spectral channel for in-vivo confocal microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6432). [643206] https://doi.org/10.1117/12.698697

Design of a multi-spectral channel for in-vivo confocal microscopy. / Makhlouf, Houssine; Tanbakuchi, Anthony A.; Rouse, Andrew R; Gmitro, Arthur F.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6432 2007. 643206.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Makhlouf, H, Tanbakuchi, AA, Rouse, AR & Gmitro, AF 2007, Design of a multi-spectral channel for in-vivo confocal microscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6432, 643206, Endoscopic Microscopy II, San Jose, CA, United States, 1/21/07. https://doi.org/10.1117/12.698697
Makhlouf H, Tanbakuchi AA, Rouse AR, Gmitro AF. Design of a multi-spectral channel for in-vivo confocal microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6432. 2007. 643206 https://doi.org/10.1117/12.698697
Makhlouf, Houssine ; Tanbakuchi, Anthony A. ; Rouse, Andrew R ; Gmitro, Arthur F. / Design of a multi-spectral channel for in-vivo confocal microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6432 2007.
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