Design of affinity membrane chromatographic columns

A. Tejeda-Mansir, J. M. Juvera, I. Magaña, Roberto Z Guzman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A method for affinity membrane column design, based on the analytical solution of the Thomas model for frontal analysis in membrane column adsorption, was developed. The method permits to find the operating conditions to reach a 93.5% of the column capacity as operating capacity, using a sharpness restriction for the system breakthrough curve. The solution of the model is presented in a graphic form and can be used in a wide range of operational conditions, provided that four design restrictions are fulfilled. The application of the method was illustrated using experimental data and a simple procedure. The implications of the results on the design and optimization of affinity membrane chromatographic columns are discussed.

Original languageEnglish (US)
Pages (from-to)115-119
Number of pages5
JournalBioprocess Engineering
Volume19
Issue number2
DOIs
StatePublished - 1998

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Membranes
Adsorption
Thomas' solution

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Chemical Engineering(all)
  • Bioengineering

Cite this

Design of affinity membrane chromatographic columns. / Tejeda-Mansir, A.; Juvera, J. M.; Magaña, I.; Guzman, Roberto Z.

In: Bioprocess Engineering, Vol. 19, No. 2, 1998, p. 115-119.

Research output: Contribution to journalArticle

Tejeda-Mansir, A, Juvera, JM, Magaña, I & Guzman, RZ 1998, 'Design of affinity membrane chromatographic columns', Bioprocess Engineering, vol. 19, no. 2, pp. 115-119. https://doi.org/10.1007/s004490050491
Tejeda-Mansir, A. ; Juvera, J. M. ; Magaña, I. ; Guzman, Roberto Z. / Design of affinity membrane chromatographic columns. In: Bioprocess Engineering. 1998 ; Vol. 19, No. 2. pp. 115-119.
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