Destripeptide insulin-like growth factor-I in milk from bovine somatotropin-treated cows

Grant T. Shimamoto, John C. Byatt, Michael G. Jennings, Patricia G. Comens-Keller, Robert J Collier

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Total somatomedins from milk of bovine somatotropin-treated cows were isolated and characterized to determine the relative amount of the three amino acid N-terminally truncated form of IGF-I (destripeptide IGF-I). The somatomedin fraction was isolated using organic solvent and solid-phase extractions followed by preparative reverse phase HPLC and affinity chromatography. The overall yield of IGF-I was 28%, and destripeptide IGF-I was recovered with similar efficiency. The isolated somatomedins were resolved by capillary zonal electrophoresis and identified using recombinant somatomedin standards. The concentration of destripeptide IGF-I relative to full length IGF-I was determined by amino terminal sequencing and by bioassay. Results from these experiments indicated that the level of destripeptide IGF-I in milk from somatotropin-treated cows was less than 3% of the IGF-I concentration. Destripeptide IGF-I is therefore a minor component of the somatomedins present in milk from treated cows and does not contribute significantly to the proliferative activity of this milk.

Original languageEnglish (US)
Pages (from-to)296-300
Number of pages5
JournalPediatric Research
Volume32
Issue number3
StatePublished - 1992
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor I
Milk
Somatomedins
bovine growth hormone
Solid Phase Extraction
Capillary Electrophoresis
Reverse-Phase Chromatography
Affinity Chromatography
Biological Assay
Growth Hormone
High Pressure Liquid Chromatography
Amino Acids

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Shimamoto, G. T., Byatt, J. C., Jennings, M. G., Comens-Keller, P. G., & Collier, R. J. (1992). Destripeptide insulin-like growth factor-I in milk from bovine somatotropin-treated cows. Pediatric Research, 32(3), 296-300.

Destripeptide insulin-like growth factor-I in milk from bovine somatotropin-treated cows. / Shimamoto, Grant T.; Byatt, John C.; Jennings, Michael G.; Comens-Keller, Patricia G.; Collier, Robert J.

In: Pediatric Research, Vol. 32, No. 3, 1992, p. 296-300.

Research output: Contribution to journalArticle

Shimamoto, GT, Byatt, JC, Jennings, MG, Comens-Keller, PG & Collier, RJ 1992, 'Destripeptide insulin-like growth factor-I in milk from bovine somatotropin-treated cows', Pediatric Research, vol. 32, no. 3, pp. 296-300.
Shimamoto, Grant T. ; Byatt, John C. ; Jennings, Michael G. ; Comens-Keller, Patricia G. ; Collier, Robert J. / Destripeptide insulin-like growth factor-I in milk from bovine somatotropin-treated cows. In: Pediatric Research. 1992 ; Vol. 32, No. 3. pp. 296-300.
@article{2f3ece4cf9af4dbeb637b5ca4fc76181,
title = "Destripeptide insulin-like growth factor-I in milk from bovine somatotropin-treated cows",
abstract = "Total somatomedins from milk of bovine somatotropin-treated cows were isolated and characterized to determine the relative amount of the three amino acid N-terminally truncated form of IGF-I (destripeptide IGF-I). The somatomedin fraction was isolated using organic solvent and solid-phase extractions followed by preparative reverse phase HPLC and affinity chromatography. The overall yield of IGF-I was 28{\%}, and destripeptide IGF-I was recovered with similar efficiency. The isolated somatomedins were resolved by capillary zonal electrophoresis and identified using recombinant somatomedin standards. The concentration of destripeptide IGF-I relative to full length IGF-I was determined by amino terminal sequencing and by bioassay. Results from these experiments indicated that the level of destripeptide IGF-I in milk from somatotropin-treated cows was less than 3{\%} of the IGF-I concentration. Destripeptide IGF-I is therefore a minor component of the somatomedins present in milk from treated cows and does not contribute significantly to the proliferative activity of this milk.",
author = "Shimamoto, {Grant T.} and Byatt, {John C.} and Jennings, {Michael G.} and Comens-Keller, {Patricia G.} and Collier, {Robert J}",
year = "1992",
language = "English (US)",
volume = "32",
pages = "296--300",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Destripeptide insulin-like growth factor-I in milk from bovine somatotropin-treated cows

AU - Shimamoto, Grant T.

AU - Byatt, John C.

AU - Jennings, Michael G.

AU - Comens-Keller, Patricia G.

AU - Collier, Robert J

PY - 1992

Y1 - 1992

N2 - Total somatomedins from milk of bovine somatotropin-treated cows were isolated and characterized to determine the relative amount of the three amino acid N-terminally truncated form of IGF-I (destripeptide IGF-I). The somatomedin fraction was isolated using organic solvent and solid-phase extractions followed by preparative reverse phase HPLC and affinity chromatography. The overall yield of IGF-I was 28%, and destripeptide IGF-I was recovered with similar efficiency. The isolated somatomedins were resolved by capillary zonal electrophoresis and identified using recombinant somatomedin standards. The concentration of destripeptide IGF-I relative to full length IGF-I was determined by amino terminal sequencing and by bioassay. Results from these experiments indicated that the level of destripeptide IGF-I in milk from somatotropin-treated cows was less than 3% of the IGF-I concentration. Destripeptide IGF-I is therefore a minor component of the somatomedins present in milk from treated cows and does not contribute significantly to the proliferative activity of this milk.

AB - Total somatomedins from milk of bovine somatotropin-treated cows were isolated and characterized to determine the relative amount of the three amino acid N-terminally truncated form of IGF-I (destripeptide IGF-I). The somatomedin fraction was isolated using organic solvent and solid-phase extractions followed by preparative reverse phase HPLC and affinity chromatography. The overall yield of IGF-I was 28%, and destripeptide IGF-I was recovered with similar efficiency. The isolated somatomedins were resolved by capillary zonal electrophoresis and identified using recombinant somatomedin standards. The concentration of destripeptide IGF-I relative to full length IGF-I was determined by amino terminal sequencing and by bioassay. Results from these experiments indicated that the level of destripeptide IGF-I in milk from somatotropin-treated cows was less than 3% of the IGF-I concentration. Destripeptide IGF-I is therefore a minor component of the somatomedins present in milk from treated cows and does not contribute significantly to the proliferative activity of this milk.

UR - http://www.scopus.com/inward/record.url?scp=0026731108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026731108&partnerID=8YFLogxK

M3 - Article

VL - 32

SP - 296

EP - 300

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 3

ER -