Destruction of preantral follicles in adult rats by 4-vinyl-1-cyclohexene diepoxide

Jodi A. Flaws, Julie K. Doerr, I. Glenn Sipes, Patricia B Hoyer

Research output: Contribution to journalArticle

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Abstract

4-vinyl-1-cyclohexene diepoxide (VCD) is known to destroy oocytes in ovaries of immature rats. Since ovaries functionally differ between immature and adult animals, we examined the effect of VCD on oocytes in adult rats. Adult (58 days) and immature (28 days) rats were injected daily (30 days) with vehicle or VCD. Each group contained 10 rats. During this time, cyclicity was determined daily by vaginal cytology. Animals were terminated on day 31 and tissues were collected. Oocytes were counted; livers, spleens, and uteri were weighed. VCD reduced the number of regular estrous cycles/30 days in adults, but not immature rats (n = 20, P < 0.05). VCD reduced the number of oocytes in adult and immature rats (n = 20, P < 0.05). Liver, spleen, or ovarian weights were not affected by VCD in either group. VCD reduced ruterine weight in adult (n = 20, P < 0.05) but not in immature rats. These results demonstrate that VCD decreases uterine weight in adult rats and as with immature rats, selectively destroys oocytes in ovaries of adults.

Original languageEnglish (US)
Pages (from-to)509-514
Number of pages6
JournalReproductive Toxicology
Volume8
Issue number6
DOIs
StatePublished - 1994

Fingerprint

Rats
Oocytes
Ovary
Weights and Measures
Liver
Animals
Spleen
Cytology
4-vinyl-1-cyclohexene dioxide
cyclohexene
Estrous Cycle
Periodicity
Uterus
Cell Biology
Tissue

Keywords

  • adult rats
  • follicle
  • infertility
  • ovarian toxicity
  • uterine toxicity
  • VCD

ASJC Scopus subject areas

  • Toxicology

Cite this

Destruction of preantral follicles in adult rats by 4-vinyl-1-cyclohexene diepoxide. / Flaws, Jodi A.; Doerr, Julie K.; Sipes, I. Glenn; Hoyer, Patricia B.

In: Reproductive Toxicology, Vol. 8, No. 6, 1994, p. 509-514.

Research output: Contribution to journalArticle

Flaws, Jodi A. ; Doerr, Julie K. ; Sipes, I. Glenn ; Hoyer, Patricia B. / Destruction of preantral follicles in adult rats by 4-vinyl-1-cyclohexene diepoxide. In: Reproductive Toxicology. 1994 ; Vol. 8, No. 6. pp. 509-514.
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