Detachment of captured cancer cells under flow acceleration in a bio-functionalized microchannel

Luthur Siu Lun Cheung, Xiangjun Zheng, Ashley Stopa, James C Baygents, Roberto Z Guzman, Joyce Schroeder, Ronald L Heimark, Yitshak Zohar

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Attachment, deformation and detachment of N-cadherin expressing prostate and breast cancer cell lines in a functionalized microchannel under hydrodynamic loading have been studied. N-cadherin antibodies are immobilized on the microchannel surface to capture the target cancer cells, PC3N and MDA-MB-231-N, from a homogeneous cell suspension. Although difficult, a significant fraction of moving cells can be captured under a low flow rate. More than 90% of the target cells are captured after a certain incubation time under no flow condition. The mechanical response of a captured cancer cell to hydrodynamic flow field is investigated and, in particular, the effect of flow acceleration is examined. The observed cell deformation is dramatic under low acceleration, but is negligible under high acceleration. Consequently, the detachment of captured cells depends on both flow rate and flow acceleration. The flow rate required for cell detachment is a random variable that can be described by a log-normal distribution. Two flow acceleration limits have been identified for proper scaling of the flow rate required to detach captured cells. A time constant for the mechanical response of a captured cell, on the order of 1 min, has been identified for scaling the flow acceleration. Based on these acceleration limits and time constant, an exponential-like empirical model is proposed to predict the flow rate required for cell detachment as a function of flow acceleration.

Original languageEnglish (US)
Pages (from-to)1721-1731
Number of pages11
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume9
Issue number12
DOIs
StatePublished - 2009

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Microchannels
Cells
Flow rate
Neoplasms
Cadherins
Hydrodynamics
Immobilized Antibodies
Normal distribution
Random variables
Antibodies
Flow fields
Suspensions
Normal Distribution
Prostatic Neoplasms
Breast Neoplasms
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

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title = "Detachment of captured cancer cells under flow acceleration in a bio-functionalized microchannel",
abstract = "Attachment, deformation and detachment of N-cadherin expressing prostate and breast cancer cell lines in a functionalized microchannel under hydrodynamic loading have been studied. N-cadherin antibodies are immobilized on the microchannel surface to capture the target cancer cells, PC3N and MDA-MB-231-N, from a homogeneous cell suspension. Although difficult, a significant fraction of moving cells can be captured under a low flow rate. More than 90{\%} of the target cells are captured after a certain incubation time under no flow condition. The mechanical response of a captured cancer cell to hydrodynamic flow field is investigated and, in particular, the effect of flow acceleration is examined. The observed cell deformation is dramatic under low acceleration, but is negligible under high acceleration. Consequently, the detachment of captured cells depends on both flow rate and flow acceleration. The flow rate required for cell detachment is a random variable that can be described by a log-normal distribution. Two flow acceleration limits have been identified for proper scaling of the flow rate required to detach captured cells. A time constant for the mechanical response of a captured cell, on the order of 1 min, has been identified for scaling the flow acceleration. Based on these acceleration limits and time constant, an exponential-like empirical model is proposed to predict the flow rate required for cell detachment as a function of flow acceleration.",
author = "Cheung, {Luthur Siu Lun} and Xiangjun Zheng and Ashley Stopa and Baygents, {James C} and Guzman, {Roberto Z} and Joyce Schroeder and Heimark, {Ronald L} and Yitshak Zohar",
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AU - Cheung, Luthur Siu Lun

AU - Zheng, Xiangjun

AU - Stopa, Ashley

AU - Baygents, James C

AU - Guzman, Roberto Z

AU - Schroeder, Joyce

AU - Heimark, Ronald L

AU - Zohar, Yitshak

PY - 2009

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N2 - Attachment, deformation and detachment of N-cadherin expressing prostate and breast cancer cell lines in a functionalized microchannel under hydrodynamic loading have been studied. N-cadherin antibodies are immobilized on the microchannel surface to capture the target cancer cells, PC3N and MDA-MB-231-N, from a homogeneous cell suspension. Although difficult, a significant fraction of moving cells can be captured under a low flow rate. More than 90% of the target cells are captured after a certain incubation time under no flow condition. The mechanical response of a captured cancer cell to hydrodynamic flow field is investigated and, in particular, the effect of flow acceleration is examined. The observed cell deformation is dramatic under low acceleration, but is negligible under high acceleration. Consequently, the detachment of captured cells depends on both flow rate and flow acceleration. The flow rate required for cell detachment is a random variable that can be described by a log-normal distribution. Two flow acceleration limits have been identified for proper scaling of the flow rate required to detach captured cells. A time constant for the mechanical response of a captured cell, on the order of 1 min, has been identified for scaling the flow acceleration. Based on these acceleration limits and time constant, an exponential-like empirical model is proposed to predict the flow rate required for cell detachment as a function of flow acceleration.

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