Detecting subsecond dopamine release with fast-scan cyclic voltammetry in vivo

Donita L. Robinson, B. Jill Venton, Michael L.A.V. Heien, R. Mark Wightman

Research output: Contribution to journalArticlepeer-review

373 Scopus citations

Abstract

Background: Dopamine is a potent neuromodulator in the brain, influencing a variety of motivated behaviors and involved in several neurologic diseases. Measurements of extracellular dopamine in the brains of experimental animals have traditionally focused on a tonic timescale (minutes to hours). However, dopamine concentrations are now known to fluctuate on a phasic timescale (subseconds to seconds). Approach: Fast-scan cyclic voltammetry provides analytical chemical measurements of phasic dopamine signals in the rat brain. Content: Procedural aspects of the technique are discussed, with regard to appropriate use and in comparison with other methods. Finally, examples of data collected using fast-scan cyclic voltammetry are summarized, including naturally occurring dopamine transients and signals arising from electrical stimulation of dopamine neurons. Summary: Fast-scan cyclic voltammetry offers real-time measurements of changes in extracellular dopamine concentrations in vivo. With its subsecond time resolution, micrometer-dimension spatial resolution, and chemical selectivity, it is the most suitable technique currently available to measure transient concentration changes of dopamine.

Original languageEnglish (US)
Pages (from-to)1763-1773
Number of pages11
JournalClinical chemistry
Volume49
Issue number10
DOIs
StatePublished - Oct 1 2003

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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