Detecting subsecond dopamine release with fast-scan cyclic voltammetry in vivo

Donita L. Robinson, B. Jill Venton, Michael L Heien, R. Mark Wightman

Research output: Contribution to journalArticle

348 Citations (Scopus)

Abstract

Background: Dopamine is a potent neuromodulator in the brain, influencing a variety of motivated behaviors and involved in several neurologic diseases. Measurements of extracellular dopamine in the brains of experimental animals have traditionally focused on a tonic timescale (minutes to hours). However, dopamine concentrations are now known to fluctuate on a phasic timescale (subseconds to seconds). Approach: Fast-scan cyclic voltammetry provides analytical chemical measurements of phasic dopamine signals in the rat brain. Content: Procedural aspects of the technique are discussed, with regard to appropriate use and in comparison with other methods. Finally, examples of data collected using fast-scan cyclic voltammetry are summarized, including naturally occurring dopamine transients and signals arising from electrical stimulation of dopamine neurons. Summary: Fast-scan cyclic voltammetry offers real-time measurements of changes in extracellular dopamine concentrations in vivo. With its subsecond time resolution, micrometer-dimension spatial resolution, and chemical selectivity, it is the most suitable technique currently available to measure transient concentration changes of dopamine.

Original languageEnglish (US)
Pages (from-to)1763-1773
Number of pages11
JournalClinical Chemistry
Volume49
Issue number10
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Cyclic voltammetry
Dopamine
Brain
Dopaminergic Neurons
Nervous System Diseases
Electric Stimulation
Neurotransmitter Agents
Time measurement
Neurons
Rats
Animals

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Detecting subsecond dopamine release with fast-scan cyclic voltammetry in vivo. / Robinson, Donita L.; Venton, B. Jill; Heien, Michael L; Wightman, R. Mark.

In: Clinical Chemistry, Vol. 49, No. 10, 01.10.2003, p. 1763-1773.

Research output: Contribution to journalArticle

Robinson, Donita L. ; Venton, B. Jill ; Heien, Michael L ; Wightman, R. Mark. / Detecting subsecond dopamine release with fast-scan cyclic voltammetry in vivo. In: Clinical Chemistry. 2003 ; Vol. 49, No. 10. pp. 1763-1773.
@article{21125f01c3a34fc891ddbbf1d51b01f2,
title = "Detecting subsecond dopamine release with fast-scan cyclic voltammetry in vivo",
abstract = "Background: Dopamine is a potent neuromodulator in the brain, influencing a variety of motivated behaviors and involved in several neurologic diseases. Measurements of extracellular dopamine in the brains of experimental animals have traditionally focused on a tonic timescale (minutes to hours). However, dopamine concentrations are now known to fluctuate on a phasic timescale (subseconds to seconds). Approach: Fast-scan cyclic voltammetry provides analytical chemical measurements of phasic dopamine signals in the rat brain. Content: Procedural aspects of the technique are discussed, with regard to appropriate use and in comparison with other methods. Finally, examples of data collected using fast-scan cyclic voltammetry are summarized, including naturally occurring dopamine transients and signals arising from electrical stimulation of dopamine neurons. Summary: Fast-scan cyclic voltammetry offers real-time measurements of changes in extracellular dopamine concentrations in vivo. With its subsecond time resolution, micrometer-dimension spatial resolution, and chemical selectivity, it is the most suitable technique currently available to measure transient concentration changes of dopamine.",
author = "Robinson, {Donita L.} and Venton, {B. Jill} and Heien, {Michael L} and Wightman, {R. Mark}",
year = "2003",
month = "10",
day = "1",
doi = "10.1373/49.10.1763",
language = "English (US)",
volume = "49",
pages = "1763--1773",
journal = "Clinical Chemistry",
issn = "0009-9147",
publisher = "American Association for Clinical Chemistry Inc.",
number = "10",

}

TY - JOUR

T1 - Detecting subsecond dopamine release with fast-scan cyclic voltammetry in vivo

AU - Robinson, Donita L.

AU - Venton, B. Jill

AU - Heien, Michael L

AU - Wightman, R. Mark

PY - 2003/10/1

Y1 - 2003/10/1

N2 - Background: Dopamine is a potent neuromodulator in the brain, influencing a variety of motivated behaviors and involved in several neurologic diseases. Measurements of extracellular dopamine in the brains of experimental animals have traditionally focused on a tonic timescale (minutes to hours). However, dopamine concentrations are now known to fluctuate on a phasic timescale (subseconds to seconds). Approach: Fast-scan cyclic voltammetry provides analytical chemical measurements of phasic dopamine signals in the rat brain. Content: Procedural aspects of the technique are discussed, with regard to appropriate use and in comparison with other methods. Finally, examples of data collected using fast-scan cyclic voltammetry are summarized, including naturally occurring dopamine transients and signals arising from electrical stimulation of dopamine neurons. Summary: Fast-scan cyclic voltammetry offers real-time measurements of changes in extracellular dopamine concentrations in vivo. With its subsecond time resolution, micrometer-dimension spatial resolution, and chemical selectivity, it is the most suitable technique currently available to measure transient concentration changes of dopamine.

AB - Background: Dopamine is a potent neuromodulator in the brain, influencing a variety of motivated behaviors and involved in several neurologic diseases. Measurements of extracellular dopamine in the brains of experimental animals have traditionally focused on a tonic timescale (minutes to hours). However, dopamine concentrations are now known to fluctuate on a phasic timescale (subseconds to seconds). Approach: Fast-scan cyclic voltammetry provides analytical chemical measurements of phasic dopamine signals in the rat brain. Content: Procedural aspects of the technique are discussed, with regard to appropriate use and in comparison with other methods. Finally, examples of data collected using fast-scan cyclic voltammetry are summarized, including naturally occurring dopamine transients and signals arising from electrical stimulation of dopamine neurons. Summary: Fast-scan cyclic voltammetry offers real-time measurements of changes in extracellular dopamine concentrations in vivo. With its subsecond time resolution, micrometer-dimension spatial resolution, and chemical selectivity, it is the most suitable technique currently available to measure transient concentration changes of dopamine.

UR - http://www.scopus.com/inward/record.url?scp=0141615741&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141615741&partnerID=8YFLogxK

U2 - 10.1373/49.10.1763

DO - 10.1373/49.10.1763

M3 - Article

C2 - 14500617

AN - SCOPUS:0141615741

VL - 49

SP - 1763

EP - 1773

JO - Clinical Chemistry

JF - Clinical Chemistry

SN - 0009-9147

IS - 10

ER -