Detection and genotyping of human papillomaviruses from archival formalin-fixed tissue samples

Koenraad Van Doorslaer, Zigui Chen, Alison A. McBride

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Pathology departments routinely process and store formalin-fixed, paraffinembedded (FFPE) tissue samples for clinical diagnosis. These collections often contain decades’ worth of samples and represent a treasure trove of specimens that can be analyzed for retrospective epidemiological studies, diagnostics, and pathogen discovery. Accurate amplification and sequencing of DNA from these samples is critical for the usability of these FFPE samples. Here we present a collection of protocols that describe extraction of DNA from FFPE tissues, PCR amplification of human papillomavirus DNA, and subsequent genotyping of the infecting virus.

Original languageEnglish (US)
Pages (from-to)14B.9.1-14B.9.20
JournalCurrent Protocols in Microbiology
Volume2016
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Keywords

  • Cancer
  • Cervix
  • Degenerate primers
  • FFPE
  • Formalin
  • HPV
  • Neoplasia
  • Papillomavirus
  • PCR
  • Sequencing

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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