Detection of 8-oxo-2'-deoxyguanosine, a marker of oxidative DNA damage, in culture medium from human mesothelial cells exposed to crocidolite asbestos

Qin Chen, Joanne Marsh, Bruce Ames, Brooke Mossman

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Crocidolite asbestos is associated with the development of mesothelioma. Although chromosomal changes have been documented in mesothelial cells, the mechanisms of interaction of crocidolite with DNA remain obscure. Since human mesothelial cells are exquisitely sensitive to asbestos, oxidative DNA damage was measured in an asbestos-exposed human mesothelial cell line (MET5A) by assaying oxidized guanine bases [8-oxo-2'-deoxyguanosine (oxo8dG), 8-oxoguanine (oxo8G), and 8-oxoguanosine (oxo8Gua)] excreted into the spent culture medium after DNA repair or turnover. At growth inhibitory, but not cytolytic concentrations, asbestos caused significant elevation of all bases in the spent medium over a 48-h period. In contrast, riebeckite, a chemically similar, nonfibrous analog of crocidolite did not cause increased adduct release. Results show that oxidative RNA and DNA bases are produced in response to asbestos in target cells of asbestos-induced cancers.

Original languageEnglish (US)
Pages (from-to)2525-2527
Number of pages3
JournalCarcinogenesis
Volume17
Issue number11
StatePublished - Nov 1996
Externally publishedYes

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Crocidolite Asbestos
Asbestos
DNA Damage
Culture Media
DNA
Mesothelioma
Guanine
Cell Communication
DNA Repair
8-oxo-7-hydrodeoxyguanosine
RNA
Cell Line
Growth
Neoplasms

ASJC Scopus subject areas

  • Cancer Research

Cite this

Detection of 8-oxo-2'-deoxyguanosine, a marker of oxidative DNA damage, in culture medium from human mesothelial cells exposed to crocidolite asbestos. / Chen, Qin; Marsh, Joanne; Ames, Bruce; Mossman, Brooke.

In: Carcinogenesis, Vol. 17, No. 11, 11.1996, p. 2525-2527.

Research output: Contribution to journalArticle

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abstract = "Crocidolite asbestos is associated with the development of mesothelioma. Although chromosomal changes have been documented in mesothelial cells, the mechanisms of interaction of crocidolite with DNA remain obscure. Since human mesothelial cells are exquisitely sensitive to asbestos, oxidative DNA damage was measured in an asbestos-exposed human mesothelial cell line (MET5A) by assaying oxidized guanine bases [8-oxo-2'-deoxyguanosine (oxo8dG), 8-oxoguanine (oxo8G), and 8-oxoguanosine (oxo8Gua)] excreted into the spent culture medium after DNA repair or turnover. At growth inhibitory, but not cytolytic concentrations, asbestos caused significant elevation of all bases in the spent medium over a 48-h period. In contrast, riebeckite, a chemically similar, nonfibrous analog of crocidolite did not cause increased adduct release. Results show that oxidative RNA and DNA bases are produced in response to asbestos in target cells of asbestos-induced cancers.",
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