Detection of Alkaline Phosphatase Enzyme Activity with a CatalyCEST MRI Biosensor

Iman Daryaei, Mahsa Mohammadebrahim Ghaffari, Kyle M. Jones, Mark D. Pagel

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Responsive CEST MRI biosensors offer good sensitivity and excellent specificity for detection of biomarkers with great potential for clinical translation. We report the application of fosfosal, a phosphorylated form of salicylic acid, for the detection of alkaline phosphatase (AP) enzyme. We detected conversion of fosfosal to salicylic acid in the presence of the enzyme by CEST MRI. Importantly the technique was able to detect AP enzyme expressed in cells in the presence of other cell components, which improves specificity. Various isoforms of the enzyme showed different Michaelis-Menten kinetics and yet these kinetics studies indicated very efficient catalytic rates. Our results with the fosfosal biosensor encourage further in vivo studies.

Original languageEnglish (US)
Pages (from-to)857-861
Number of pages5
JournalACS Sensors
Volume1
Issue number7
DOIs
StatePublished - Jul 22 2016

Keywords

  • CATALYCEST
  • CEST MRI
  • Michaelis-Menten kinetics
  • alkaline phosphatase
  • molecular imaging

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

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