Detection of Alkaline Phosphatase Enzyme Activity with a CatalyCEST MRI Biosensor

Iman Daryaei, Mahsa Mohammadebrahim Ghaffari, Kyle M. Jones, Mark "Marty" Pagel

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Responsive CEST MRI biosensors offer good sensitivity and excellent specificity for detection of biomarkers with great potential for clinical translation. We report the application of fosfosal, a phosphorylated form of salicylic acid, for the detection of alkaline phosphatase (AP) enzyme. We detected conversion of fosfosal to salicylic acid in the presence of the enzyme by CEST MRI. Importantly the technique was able to detect AP enzyme expressed in cells in the presence of other cell components, which improves specificity. Various isoforms of the enzyme showed different Michaelis-Menten kinetics and yet these kinetics studies indicated very efficient catalytic rates. Our results with the fosfosal biosensor encourage further in vivo studies.

Original languageEnglish (US)
Pages (from-to)857-861
Number of pages5
JournalACS Sensors
Volume1
Issue number7
DOIs
StatePublished - Jul 22 2016

Fingerprint

enzyme activity
phosphatases
Phosphatases
Enzyme activity
bioinstrumentation
Biosensors
Magnetic resonance imaging
Alkaline Phosphatase
enzymes
Enzymes
Salicylic acid
Salicylic Acid
Enzyme kinetics
acids
Kinetics
biomarkers
kinetics
Biomarkers
cells
Protein Isoforms

Keywords

  • alkaline phosphatase
  • CATALYCEST
  • CEST MRI
  • Michaelis-Menten kinetics
  • molecular imaging

ASJC Scopus subject areas

  • Bioengineering
  • Fluid Flow and Transfer Processes
  • Process Chemistry and Technology
  • Instrumentation

Cite this

Detection of Alkaline Phosphatase Enzyme Activity with a CatalyCEST MRI Biosensor. / Daryaei, Iman; Mohammadebrahim Ghaffari, Mahsa; Jones, Kyle M.; Pagel, Mark "Marty".

In: ACS Sensors, Vol. 1, No. 7, 22.07.2016, p. 857-861.

Research output: Contribution to journalArticle

Daryaei, Iman ; Mohammadebrahim Ghaffari, Mahsa ; Jones, Kyle M. ; Pagel, Mark "Marty". / Detection of Alkaline Phosphatase Enzyme Activity with a CatalyCEST MRI Biosensor. In: ACS Sensors. 2016 ; Vol. 1, No. 7. pp. 857-861.
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