DETECTION OF CRYPTOSPORIDIUM FROM WASTEWATER AND FRESHWATER ENVIRONMENTS.

J. B. Rose, A. Cifrino, M. S. Madore, Charles P Gerba, Charles R Sterling, M. J. Arrowood

Research output: Chapter in Book/Report/Conference proceedingChapter

38 Citations (Scopus)

Abstract

The method developed for detection of Cryptosporidium in water including: 1) filtration with polypropylene cartridge filters, 2) elution with Tween 80/detergent, 3) concentration with Sheather's flotation, and 4) detection on membrane filters, was evaluated and used to examine wastewater and fresh water for Cryptosporidium cocyst concentrations. Recovery from the sucrose gradient step averaged 72-82%. Overall recovery efficiencies ranged from 25-82% (average 50%), but were diminished with low seeds. Concentrations were found in ranges from 60 to 52,000 oocysts/gal in treated and raw sewage. In fresh water, oocysts numbered 8 to 22,000/gal and were concentrated in rapid sand filters averagingb 59,000/gal in the backflush. Cryptosporidium may now be considered a potential waterborne parasite.

Original languageEnglish (US)
Title of host publicationWater Science and Technology
Pages233-239
Number of pages7
Volume18
Edition10
StatePublished - 1986
EventHealth-Relat Water Microbiol 1986 - Rio De Janeiro, Braz
Duration: Aug 20 1986Aug 22 1986

Other

OtherHealth-Relat Water Microbiol 1986
CityRio De Janeiro, Braz
Period8/20/868/22/86

Fingerprint

freshwater environment
filter
wastewater
detergent
sucrose
water
parasite
sewage
membrane
seed
sand
detection

ASJC Scopus subject areas

  • Water Science and Technology

Cite this

Rose, J. B., Cifrino, A., Madore, M. S., Gerba, C. P., Sterling, C. R., & Arrowood, M. J. (1986). DETECTION OF CRYPTOSPORIDIUM FROM WASTEWATER AND FRESHWATER ENVIRONMENTS. In Water Science and Technology (10 ed., Vol. 18, pp. 233-239)

DETECTION OF CRYPTOSPORIDIUM FROM WASTEWATER AND FRESHWATER ENVIRONMENTS. / Rose, J. B.; Cifrino, A.; Madore, M. S.; Gerba, Charles P; Sterling, Charles R; Arrowood, M. J.

Water Science and Technology. Vol. 18 10. ed. 1986. p. 233-239.

Research output: Chapter in Book/Report/Conference proceedingChapter

Rose, JB, Cifrino, A, Madore, MS, Gerba, CP, Sterling, CR & Arrowood, MJ 1986, DETECTION OF CRYPTOSPORIDIUM FROM WASTEWATER AND FRESHWATER ENVIRONMENTS. in Water Science and Technology. 10 edn, vol. 18, pp. 233-239, Health-Relat Water Microbiol 1986, Rio De Janeiro, Braz, 8/20/86.
Rose JB, Cifrino A, Madore MS, Gerba CP, Sterling CR, Arrowood MJ. DETECTION OF CRYPTOSPORIDIUM FROM WASTEWATER AND FRESHWATER ENVIRONMENTS. In Water Science and Technology. 10 ed. Vol. 18. 1986. p. 233-239
Rose, J. B. ; Cifrino, A. ; Madore, M. S. ; Gerba, Charles P ; Sterling, Charles R ; Arrowood, M. J. / DETECTION OF CRYPTOSPORIDIUM FROM WASTEWATER AND FRESHWATER ENVIRONMENTS. Water Science and Technology. Vol. 18 10. ed. 1986. pp. 233-239
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