Detection of Cryptosporidium in water by using polypropylene cartridge filters

C. E. Musial, M. J. Arrowood, C. R. Sterling, C. P. Gerba

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

Members of the genus Cryptosporidium are protozoan parasites that cause gastroenteritis in humans and animals and appear to be spread largely by the fecal-oral route. A method was developed for the concentration and detection of Cryptosporidium oocysts in water to assess their occurrence in the environment and potential for waterborne disease transmission. This method was developed by using spun polypropylene cartridge filters. Optimal conditions for concentration, filter elution, filter porosity, and detection were determined. Fluoresceinated monoclonal antibodies were used for oocyst detection. Experiments also were conducted to study the effect of flow rate, low oocyst numbers, and the addition of detergents on recovery and retention of oocysts. The method that was developed was sensitive enough to detect oocysts at levels of less than 1 per liter. Using this method, we isolated Cryptosporidium oocysts from secondarily treated sewage.

Original languageEnglish (US)
Pages (from-to)687-692
Number of pages6
JournalApplied and environmental microbiology
Volume53
Issue number4
StatePublished - Jan 1 1987

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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