Detection of enterotoxigenic escherichia coli by DNA colony hybridization

S. L. Moseley, I. Huq, A. R M A Alim, Magdalene "Maggie" So, M. Samadpour-Motalebi, S. Falkow

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

A method for detecting large numbers of isolates of enterotoxigenic Escherichia coli is described in which the genes encoding the enterotoxins are detected, rather than the toxins themselves. Radiolabeled fragments of DNA encoding the heat-labile (LT) or heatstable (ST) toxins were used as hybridization probes for homologous DNA sequences in E. coli colonies grown and lysed in situ on nitrocellulose filters. The LT probe detected all of 31 E. coli strains probducing ST and LT or only LT, while the ST probe detected 12 of 17 strains producing only ST and three of 26 strains producing ST and LT. These results suggest that the LTs produced by different isolates of E. coli are homologous and that human isolates of E. coli produce at least two heterologous STs detectable in the infant mouse assay. The hybridization method also detected the presence of enterotoxigenic E. coli in bacterial growth in directly spotted stools from patients with acute diarrhea.

Original languageEnglish (US)
Pages (from-to)892-898
Number of pages7
JournalJournal of Infectious Diseases
Volume142
Issue number6
StatePublished - 1980
Externally publishedYes

Fingerprint

Enterotoxigenic Escherichia coli
Escherichia coli
DNA
Collodion
Enterotoxins
Sequence Homology
Diarrhea
Hot Temperature
Growth
Genes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Moseley, S. L., Huq, I., Alim, A. R. M. A., So, M. M., Samadpour-Motalebi, M., & Falkow, S. (1980). Detection of enterotoxigenic escherichia coli by DNA colony hybridization. Journal of Infectious Diseases, 142(6), 892-898.

Detection of enterotoxigenic escherichia coli by DNA colony hybridization. / Moseley, S. L.; Huq, I.; Alim, A. R M A; So, Magdalene "Maggie"; Samadpour-Motalebi, M.; Falkow, S.

In: Journal of Infectious Diseases, Vol. 142, No. 6, 1980, p. 892-898.

Research output: Contribution to journalArticle

Moseley, SL, Huq, I, Alim, ARMA, So, MM, Samadpour-Motalebi, M & Falkow, S 1980, 'Detection of enterotoxigenic escherichia coli by DNA colony hybridization', Journal of Infectious Diseases, vol. 142, no. 6, pp. 892-898.
Moseley SL, Huq I, Alim ARMA, So MM, Samadpour-Motalebi M, Falkow S. Detection of enterotoxigenic escherichia coli by DNA colony hybridization. Journal of Infectious Diseases. 1980;142(6):892-898.
Moseley, S. L. ; Huq, I. ; Alim, A. R M A ; So, Magdalene "Maggie" ; Samadpour-Motalebi, M. ; Falkow, S. / Detection of enterotoxigenic escherichia coli by DNA colony hybridization. In: Journal of Infectious Diseases. 1980 ; Vol. 142, No. 6. pp. 892-898.
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