Detection of ethanol consumption by ELISA assay measurement of acetaldehyde adducts in murine hair

Ronald R Watson, David Solkoff, James Y. Wang, Keifong Seeto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Acetaldehyde (AA), a principal metabolite of ethanol, reacts with proteins to form protein-AA adducts in vivo and in vitro. We studied three different protein-AA adducts produced m vitro, as well as hair keratin-AA adducts from ethanol-fed and/or cocaine-injected mice. An enzyme linked immunosorbent assay (ELISA) was developed that detected the stable protein- AA adducts m protein isolated from hair. Cocaine injection had little effect on hair AA adduct formation. The indirect ELISA assay showed significantly increased levels of keratin-AA adducts an hair from mice fed ethanol for 8 weeks. Hair collection was nominvasive and the AA-protein adducts were stable. Therefore, this indirect ELISA assay could be further developed as a biochemical parameter for alcoholism in the clinical setting.

Original languageEnglish (US)
Pages (from-to)279-284
Number of pages6
JournalAlcohol
Volume16
Issue number4
DOIs
StatePublished - Nov 1998

Fingerprint

Immunosorbents
Acetaldehyde
alcoholism
Hair
Assays
Ethanol
Enzyme-Linked Immunosorbent Assay
Enzymes
Proteins
Cocaine
Hair-Specific Keratins
Metabolites
Keratins
Alcoholism
Injections

Keywords

  • Acetaldehyde
  • Alcohol Hair
  • ELISA Adducts

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neuroscience(all)
  • Toxicology
  • Health(social science)

Cite this

Detection of ethanol consumption by ELISA assay measurement of acetaldehyde adducts in murine hair. / Watson, Ronald R; Solkoff, David; Wang, James Y.; Seeto, Keifong.

In: Alcohol, Vol. 16, No. 4, 11.1998, p. 279-284.

Research output: Contribution to journalArticle

Watson, Ronald R ; Solkoff, David ; Wang, James Y. ; Seeto, Keifong. / Detection of ethanol consumption by ELISA assay measurement of acetaldehyde adducts in murine hair. In: Alcohol. 1998 ; Vol. 16, No. 4. pp. 279-284.
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