Detection of ethanol consumption by ELISA assay measurement of acetaldehyde adducts in murine hair

Ronald R. Watson, David Solkoff, James Y. Wang, Keifong Seeto

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Acetaldehyde (AA), a principal metabolite of ethanol, reacts with proteins to form protein-AA adducts in vivo and in vitro. We studied three different protein-AA adducts produced m vitro, as well as hair keratin-AA adducts from ethanol-fed and/or cocaine-injected mice. An enzyme linked immunosorbent assay (ELISA) was developed that detected the stable protein- AA adducts m protein isolated from hair. Cocaine injection had little effect on hair AA adduct formation. The indirect ELISA assay showed significantly increased levels of keratin-AA adducts an hair from mice fed ethanol for 8 weeks. Hair collection was nominvasive and the AA-protein adducts were stable. Therefore, this indirect ELISA assay could be further developed as a biochemical parameter for alcoholism in the clinical setting.

Original languageEnglish (US)
Pages (from-to)279-284
Number of pages6
JournalAlcohol
Volume16
Issue number4
DOIs
StatePublished - Nov 1 1998

Keywords

  • Acetaldehyde
  • Alcohol Hair
  • ELISA Adducts

ASJC Scopus subject areas

  • Health(social science)
  • Biochemistry
  • Toxicology
  • Neurology
  • Behavioral Neuroscience

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