Detection of glutathione S-transferase isozymes after electrofocusing in polyacrylamide gels

William C. Kenney, Thomas D Boyer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A procedure for the rapid identification of glutathione S-transferase isozymes from rat liver in polyacrylamide gels is described. The isozymes are separated by electrofocusing and then identified by bathing the gels in a solution containing substrates and scanning the gels at the appropriate wavelength for the appearance of product. Increase in absorbance as a function of time delineates areas containing enzyme from artifacts within the gel. This technique should be useful for the identification of isozymes of glutathione S-transferase in other tissues and also other species. Also, the technique provides for rapid confirmation of homogeneity of the isozymes of glutathione S-transferase.

Original languageEnglish (US)
Pages (from-to)344-348
Number of pages5
JournalAnalytical Biochemistry
Volume116
Issue number2
DOIs
StatePublished - Sep 15 1981
Externally publishedYes

Fingerprint

Isoelectric Focusing
Glutathione Transferase
Isoenzymes
Gels
Liver
Artifacts
Rats
Tissue
Scanning
Wavelength
polyacrylamide gels
Substrates
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Detection of glutathione S-transferase isozymes after electrofocusing in polyacrylamide gels. / Kenney, William C.; Boyer, Thomas D.

In: Analytical Biochemistry, Vol. 116, No. 2, 15.09.1981, p. 344-348.

Research output: Contribution to journalArticle

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