Detection of infectious enteroviruses by an integrated cell culture-PCR procedure

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106 Citations (Scopus)

Abstract

Rapid detection of infectious enteroviruses in environmental samples was made possible by utilizing an integrated cell culture-reverse transcriptase PCR approach. By this method, the presence of infectious enterovirus was confirmed within 24 h, compared with ≥3 days by cell culture alone. The combined methodology eliminated typical problems normally associated with direct reverse transcriptase PCR by increasing the equivalent volume of environmental sample examined and reducing the effects of inhibitory compounds.

Original languageEnglish (US)
Pages (from-to)1424-1427
Number of pages4
JournalApplied and Environmental Microbiology
Volume62
Issue number4
StatePublished - Apr 1996

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Enterovirus
Reverse Transcriptase Polymerase Chain Reaction
cell culture
Cell Culture Techniques
reverse transcriptase polymerase chain reaction
Polymerase Chain Reaction
sampling
methodology
enterovirus
detection
effect
method

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

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title = "Detection of infectious enteroviruses by an integrated cell culture-PCR procedure",
abstract = "Rapid detection of infectious enteroviruses in environmental samples was made possible by utilizing an integrated cell culture-reverse transcriptase PCR approach. By this method, the presence of infectious enterovirus was confirmed within 24 h, compared with ≥3 days by cell culture alone. The combined methodology eliminated typical problems normally associated with direct reverse transcriptase PCR by increasing the equivalent volume of environmental sample examined and reducing the effects of inhibitory compounds.",
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