Detection of multiple gains and losses of genetic material in ten glioma cell lines by comparative genomic hybridization

Gayatry Mohapatra, Dong H. Kim, Burt G. Feuerstein

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

A protocol for comparative genomic hybridization by use of nucleotides directly labeled with fluorochromes was used to map regions of deletion and amplification in ten glioma cell lines. The protocol greatly reduced experimental artifacts. We detected several genetic aberrations, including whole chromosome loss and gain, partial loss and gain, possible isochromosome, and higher level DNA amplification. The most frequent losses (in order of frequency) occurred on chromosomes 10, 18, 13, 11, 9, 14, 4, 6, 1, and X. The most common gain occurred on chromosome 7. Several sites of previously known and unknown amplifications were observed. © 1995 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)86-93
Number of pages8
JournalGenes, Chromosomes and Cancer
Volume13
Issue number2
DOIs
StatePublished - Jun 1995
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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