Detection of Murine Intestinal Adenomas Using Targeted Molecular Autofluorescence

Bhaskar Banerjee, Jeffrey O. Henderson, Thomas C. Chaney, Nicholas O. Davidson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

There is a significant need for noninvasive methods of evaluating dysplastic and neoplastic lesions in the luminal gastrointestinal tract. We have optimized an approach based on autofluorescence to study dysplastic adenomatous polyps in Apcmin/+ mice. We made recordings from both normal intestinal mucosa and from polyps using a xenon lamp-based fiberoptic device. Seventy-eight polyps in 11 mice revealed an increase in mean autofluorescence intensity ratios of 1.29 ± 0.04 (72 small intestinal polyps; P < 0.0001) and 1.28 ± 0.05 (6 colon polyps; P = 0.0016). Serial measurements of autofluorescence discriminated polyps from normal mucosa with a sensitivity, verified histologically, of 95%. To understand the chemical basis for increased autofluorescence, we examined the tryptophan content of intestinal polyps and the adjacent normal mucosa in a small subset of animals. The findings revealed an increased concentration of tryptophan in polyps (990 ± 240 ng/mg) compared to normal mucosa (720 = 150 ng/mg; P = 0.03). In conclusion, these findings suggest that autofluorescence intensity increases in the setting of intestinal neoplasia and can be used to detect adenomas in the mouse intestine in real time.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalDigestive Diseases and Sciences
Volume49
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

Fingerprint

Intestinal Polyps
Polyps
Adenoma
Mucous Membrane
Tryptophan
Adenomatous Polyps
Xenon
Intestinal Mucosa
Intestines
Gastrointestinal Tract
Colon
Equipment and Supplies
Neoplasms

Keywords

  • Cancer
  • Colon
  • Dysplasia
  • Polyp

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Detection of Murine Intestinal Adenomas Using Targeted Molecular Autofluorescence. / Banerjee, Bhaskar; Henderson, Jeffrey O.; Chaney, Thomas C.; Davidson, Nicholas O.

In: Digestive Diseases and Sciences, Vol. 49, No. 1, 01.2004, p. 54-59.

Research output: Contribution to journalArticle

Banerjee, Bhaskar ; Henderson, Jeffrey O. ; Chaney, Thomas C. ; Davidson, Nicholas O. / Detection of Murine Intestinal Adenomas Using Targeted Molecular Autofluorescence. In: Digestive Diseases and Sciences. 2004 ; Vol. 49, No. 1. pp. 54-59.
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