Detection of myocardial ischemia-reperfusion injury using a fluorescent near-infrared zinc(II)-dipicolylamine probe and 99mTc glucarate

Leonie Wyffels, Brian D. Gray, Christy Barber, Koon Y. Pak, Safiyyah Forbes, Jeffrey A. Mattis, James M. Woolfenden, Zhonglin Liu

Research output: Contribution to journalArticle

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Abstract

A fluorescent zinc 2,2'-dipicolylamine coordination complex PSVue ®794 (probe 1) is known to selectively bind to phosphatidylserine exposed on the surface of apoptotic and necrotic cells. In this study, we investigated the cell death targeting properties of probe 1 in myocardial ischemia-reperfusion injury. A rat heart model of ischemia-reperfusion was used. Probe 1, control dye, or 99mTc glucarate was intravenously injected in rats subjected to 30-minute and 5-minute myocardial ischemia followed by 2-hour reperfusion. At 90 minutes or 20 hours postinjection, myocardial uptake was evaluated ex vivo by fluorescence imaging and autoradiography. Hematoxylin-eosin and cleaved caspase-3 staining was performed on myocardial sections to demonstrate the presence of ischemiareperfusion injury and apoptosis. Selective accumulation of probe 1 could be detected in the area at risk up to 20 hours postinjection. Similar topography and extent of uptake of probe 1 and 99mTc glucarate were observed at 90 minutes postinjection. Histologic analysis demonstrated the presence of necrosis, but only a few apoptotic cells could be detected. Probe 1 selectively accumulates in myocardial ischemia-reperfusion injury and is a promising cell death imaging tool.

Original languageEnglish (US)
Pages (from-to)187-196
Number of pages10
JournalMolecular Imaging
Volume11
Issue number3
DOIs
StatePublished - May 2012

Fingerprint

Myocardial Reperfusion Injury
ischemia
Reperfusion Injury
Myocardial Ischemia
Zinc
zinc
Infrared radiation
Reperfusion
probes
Cell Death
Cell death
Optical Imaging
Phosphatidylserines
Coordination Complexes
Hematoxylin
Eosine Yellowish-(YS)
Autoradiography
death
Caspase 3
rats

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Biotechnology
  • Molecular Medicine
  • Biomedical Engineering
  • Condensed Matter Physics

Cite this

Detection of myocardial ischemia-reperfusion injury using a fluorescent near-infrared zinc(II)-dipicolylamine probe and 99mTc glucarate. / Wyffels, Leonie; Gray, Brian D.; Barber, Christy; Pak, Koon Y.; Forbes, Safiyyah; Mattis, Jeffrey A.; Woolfenden, James M.; Liu, Zhonglin.

In: Molecular Imaging, Vol. 11, No. 3, 05.2012, p. 187-196.

Research output: Contribution to journalArticle

Wyffels, Leonie ; Gray, Brian D. ; Barber, Christy ; Pak, Koon Y. ; Forbes, Safiyyah ; Mattis, Jeffrey A. ; Woolfenden, James M. ; Liu, Zhonglin. / Detection of myocardial ischemia-reperfusion injury using a fluorescent near-infrared zinc(II)-dipicolylamine probe and 99mTc glucarate. In: Molecular Imaging. 2012 ; Vol. 11, No. 3. pp. 187-196.
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