Detection of myocardial viability in ischemic-reperfused rat hearts by Tc-99m sestamibi kinetics

Zhonglin Liu, Gerald Johnson, Delia Beju, Robert D. Okada

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Background. The purpose of this study was to evaluate technetium 99m sestamibi (MIBI) kinetics in assessing myocardial viability in hearts subjected to different ischemia-reperfusion treatments, resulting in graded severity of injury. Methods and Results. Sixteen isolated Krebs-Henseleit-perfused rat hearts were divided into 3 groups: control (flow, 12 mL/min; n = 5), ischemic-reperfused with glucose (IR+G, n = 6), and ischemic-reperfused without glucose (IR-G, n = 5). MIBI (11.1 mBq [300 μCi]) was infused for 60 minutes (uptake), followed by a 60-minute clearance. MIBI uptake (percent injected dose per gram) was significantly decreased in the IR+G (2.07 ± 0.31) and IR-G groups (2.03 ± 0.23; P = not significant with IR+G) compared with the control group (3.06 ± 0.25, P < .05). Fractional washout of MIBI was more rapid in the IR-G group (72.7% ± 3.9%, P < .05) than in the control (21.9% ± 1.9%) and IR+G groups (20.3% ± 1.7%). End retention (percent injected dose per gram) of MIBI in the IR-G (0.60 ± 0.12) and IR+G groups (1.60 ± 0.18) was significantly less than in the control group (2.30 ± 0.11, P < .05), respectively. The retention in the IR-G group was less than in the IR+G group (P < .05). Creatine kinase assay, triphenyltetrazolium chloride staining, and transmission electron microscopy analysis demonstrated more serious myocardial damage in the IR-G group than in the IR+G group. End MIBI activity was highly correlated with myocardial viability determined by triphenyltetrazolium chloride staining (r = 0.94; P < .05) and creatine kinase assay (r = -0.86; P < .05). Conclusions. Clearance of Tc-99m sestamibi is sensitive to metabolic states and may be used for assessment of ongoing myocardial damage.

Original languageEnglish (US)
Pages (from-to)677-686
Number of pages10
JournalJournal of Nuclear Cardiology
Volume8
Issue number6
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

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Keywords

  • Ischemia
  • Myocardium
  • Reperfusion
  • Technetium 99m sestamibi
  • Viability

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

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