Detection of pathogenic micro-organisms on children's hands and toys during play

T. Martínez-Bastidas, N. Castro-del Campo, K. D. Mena, N. Castro-del Campo, J. León-Félix, Charles P Gerba, C. Chaidez

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aims: This study aimed to determine if the children's leisure activities impact the presence of pathogens on their hands and toys. Methods & Results: To assess the microbiological hazard in playground areas, a pilot study that included 12 children was conducted. We then conducted an intervention study; children's hands and toys were washed before playing. Faecal coliforms, pathogenic bacteria and Giardia lamblia were quantified by membrane filtration, selective media and flotation techniques, respectively; rotavirus, hepatitis A and rhinovirus by RT-PCR. Pilot study results revealed faecal contamination on children's hands and toys after playing on sidewalks and in public parks. Pathogenic bacteria, hepatitis A and G. lamblia on children's hands were also found. In the intervention study, Staphylococcus aureus and Klebsiella pneumoniae were found on children's hands at concentrations up to 2·5 × 104 and 1 × 104 CFU hands-1, respectively. E. coli and Kl. pneumoniae were detected on toys (2·4 × 103 and 2·7 × 104 CFU toy-1, respectively). Salmonella spp, Serratia spp and G. lamblia cysts were also present on toys. Conclusion: Children's play activities influence microbial presence on hands and toys; the transfer seems to occur in both ways. Significance and Impact of the Study: Control strategy needs to be implemented to protect children from infectious diseases.

Original languageEnglish (US)
Pages (from-to)1668-1675
Number of pages8
JournalJournal of Applied Microbiology
Volume116
Issue number6
DOIs
StatePublished - 2014

Fingerprint

Play and Playthings
Hand
Giardia
Hepatitis A
Staphylococcal Pneumonia
Bacteria
Serratia
Rhinovirus
Giardia lamblia
Rotavirus
Leisure Activities
Klebsiella pneumoniae
Salmonella
Communicable Diseases
Cysts
Pneumonia
Escherichia coli
Polymerase Chain Reaction
Membranes

Keywords

  • Children
  • Hands
  • Human pathogens
  • Outdoor activities
  • Playgrounds
  • Toys

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

Cite this

Martínez-Bastidas, T., Castro-del Campo, N., Mena, K. D., Castro-del Campo, N., León-Félix, J., Gerba, C. P., & Chaidez, C. (2014). Detection of pathogenic micro-organisms on children's hands and toys during play. Journal of Applied Microbiology, 116(6), 1668-1675. https://doi.org/10.1111/jam.12473

Detection of pathogenic micro-organisms on children's hands and toys during play. / Martínez-Bastidas, T.; Castro-del Campo, N.; Mena, K. D.; Castro-del Campo, N.; León-Félix, J.; Gerba, Charles P; Chaidez, C.

In: Journal of Applied Microbiology, Vol. 116, No. 6, 2014, p. 1668-1675.

Research output: Contribution to journalArticle

Martínez-Bastidas, T, Castro-del Campo, N, Mena, KD, Castro-del Campo, N, León-Félix, J, Gerba, CP & Chaidez, C 2014, 'Detection of pathogenic micro-organisms on children's hands and toys during play', Journal of Applied Microbiology, vol. 116, no. 6, pp. 1668-1675. https://doi.org/10.1111/jam.12473
Martínez-Bastidas T, Castro-del Campo N, Mena KD, Castro-del Campo N, León-Félix J, Gerba CP et al. Detection of pathogenic micro-organisms on children's hands and toys during play. Journal of Applied Microbiology. 2014;116(6):1668-1675. https://doi.org/10.1111/jam.12473
Martínez-Bastidas, T. ; Castro-del Campo, N. ; Mena, K. D. ; Castro-del Campo, N. ; León-Félix, J. ; Gerba, Charles P ; Chaidez, C. / Detection of pathogenic micro-organisms on children's hands and toys during play. In: Journal of Applied Microbiology. 2014 ; Vol. 116, No. 6. pp. 1668-1675.
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