Detection of Sulfatase Enzyme Activity with a CatalyCEST MRI Contrast Agent

Sanhita Sinharay, Gabriela Fernández-Cuervo, Jasmine P. Acfalle, Mark D. Pagel

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A chemical exchange saturation transfer (CEST) MRI contrast agent has been developed that detects sulfatase enzyme activity. The agent produces a CEST signal at δ=5.0 ppm before enzyme activity, and a second CEST signal appears at δ=9.0 ppm after the enzyme cleaves a sulfate group from the agent. The comparison of the two signals improved detection of sulfatase activity.

Original languageEnglish (US)
Pages (from-to)6491-6495
Number of pages5
JournalChemistry - A European Journal
Volume22
Issue number19
DOIs
StatePublished - May 4 2016

Keywords

  • NMR imaging
  • diagnostic agents
  • enzyme kinetics
  • kinetics
  • sulfatases

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry

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