Detection of the microsporidian Enterocytozoon hepatopenaei (EHP) and Taura syndrome virus in Penaeus vannamei cultured in Venezuela

Feng-Jyu Tang-Nelson, Luis Fernando Aranguren, Patharapol Piamsomboon, Jee Eun Han, Irina Y. Maskaykina, Margeaux M. Schmidt

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The microsporidian Enterocytozoon hepatopenaei (EHP) is an intracellular parasite that has become a critical threat to the shrimp farming industry in SE Asia. This parasite replicates in the hepatopancreas and midgut, and infected shrimp exhibit reduced feeding and severely retarded growth. In this study, we describe the first case of EHP-infected Penaeus vannamei cultured in Venezuela. Its histopathology is very similar to that of SE Asia EHP, with infected shrimp showing basophilic inclusions in hepatopancreas. Upon in situ hybridization of an EHP 18S rRNA gene fragment labeled with digoxigenin, the probe reacted intensely to the basophilic inclusions within the cytoplasm of the infected cells. We also compared the nucleotide sequence similarities in 18S rRNA gene (1095-bp), in β-tubulin (583-bp) and spore wall protein (431-bp) genes between the Venezuela EHP and SE Asia isolates; and the results showed 99%, 93% and 91% identities, respectively. This suggests that the Venezuela EHP was not recently introduced from SE Asia. In a shrimp farm that was affected by EHP, Taura syndrome virus (TSV) was also detected in the P. vannamei by RT-qPCR and histological examinations. TSV is a major pathogen in penaeid shrimp aquaculture and has caused substantial economic losses during the last 2 decades, however, TSV has not been reported since 2011. This TSV was determined to be a Venezuela genotype based on the phylogenetic analysis in the capsid protein 2 gene and it showed to have sequence identities of 97% (nucleotide) and 98% (amino acid) to a Venezuela isolate found in 2005. The emergence of EHP and occurrence of TSV in the Venezuela will have a significant impact on the shrimp production if they spread to other farms. Therefore, the shrimp cultured within the country should be monitored closely for the presence of EHP and TSV.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalAquaculture
Volume480
DOIs
StatePublished - Nov 1 2017
Externally publishedYes

Fingerprint

Taura syndrome virus
Microsporidia
Litopenaeus vannamei
Venezuela
virus
shrimp
gene
parasite
hepatopancreas
farm
protein
histopathology
cytoplasm
genes
ribosomal RNA
aquaculture
Enterocytozoon
detection
parasites
spore

Keywords

  • 18S rRNA, β-tubulin gene
  • Enterocytozoon hepatopenaei (EHP)
  • Spore wall protein gene
  • Taura syndrome virus (TSV)
  • Venezuela type

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Detection of the microsporidian Enterocytozoon hepatopenaei (EHP) and Taura syndrome virus in Penaeus vannamei cultured in Venezuela. / Tang-Nelson, Feng-Jyu; Aranguren, Luis Fernando; Piamsomboon, Patharapol; Han, Jee Eun; Maskaykina, Irina Y.; Schmidt, Margeaux M.

In: Aquaculture, Vol. 480, 01.11.2017, p. 17-21.

Research output: Contribution to journalArticle

Tang-Nelson, Feng-Jyu ; Aranguren, Luis Fernando ; Piamsomboon, Patharapol ; Han, Jee Eun ; Maskaykina, Irina Y. ; Schmidt, Margeaux M. / Detection of the microsporidian Enterocytozoon hepatopenaei (EHP) and Taura syndrome virus in Penaeus vannamei cultured in Venezuela. In: Aquaculture. 2017 ; Vol. 480. pp. 17-21.
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abstract = "The microsporidian Enterocytozoon hepatopenaei (EHP) is an intracellular parasite that has become a critical threat to the shrimp farming industry in SE Asia. This parasite replicates in the hepatopancreas and midgut, and infected shrimp exhibit reduced feeding and severely retarded growth. In this study, we describe the first case of EHP-infected Penaeus vannamei cultured in Venezuela. Its histopathology is very similar to that of SE Asia EHP, with infected shrimp showing basophilic inclusions in hepatopancreas. Upon in situ hybridization of an EHP 18S rRNA gene fragment labeled with digoxigenin, the probe reacted intensely to the basophilic inclusions within the cytoplasm of the infected cells. We also compared the nucleotide sequence similarities in 18S rRNA gene (1095-bp), in β-tubulin (583-bp) and spore wall protein (431-bp) genes between the Venezuela EHP and SE Asia isolates; and the results showed 99{\%}, 93{\%} and 91{\%} identities, respectively. This suggests that the Venezuela EHP was not recently introduced from SE Asia. In a shrimp farm that was affected by EHP, Taura syndrome virus (TSV) was also detected in the P. vannamei by RT-qPCR and histological examinations. TSV is a major pathogen in penaeid shrimp aquaculture and has caused substantial economic losses during the last 2 decades, however, TSV has not been reported since 2011. This TSV was determined to be a Venezuela genotype based on the phylogenetic analysis in the capsid protein 2 gene and it showed to have sequence identities of 97{\%} (nucleotide) and 98{\%} (amino acid) to a Venezuela isolate found in 2005. The emergence of EHP and occurrence of TSV in the Venezuela will have a significant impact on the shrimp production if they spread to other farms. Therefore, the shrimp cultured within the country should be monitored closely for the presence of EHP and TSV.",
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