Determinants of differential liver-colonizing potential of variants of the MCA-38 murine colon cancer cell line

James J. Piscatelli, Stephan A. Cohen, Charles S. Berensont, Peter Lance

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We investigated factors that might contribute to the differing liver tumor colonizing potentials of MCA-38 colonic cancer cell line variants injected into the ileocolic veins of C57B1/6J mice. Non-colonizing (MCA-38 CD) cells were sensitive to lysis by hepatic ntural killer (NK) cells in vitro (51 Cr-release assay) and cells with high liver-colonizing potential (MCA-38 LD) were resistant. Following abrogation of NK activity by treatment with anti-asialoGM1, liver-colonizing ability of LD cells but not CD cells was enhanced. MCA-38 CD cells were, however, capable of initial liver colonization after ileocolic vein injection. Differing patterns of membrane sialylation may have contributed to the contrasting hepatic tumorigenicities of LD and CD cells; β-galactoside α2,6-sialyltransferase mRNA levels and activity were ∼ four-fold higher in LD than CD cells and qualitative and quantitative differences existed between their ganglioside profiles. In the MCA-38 model outlined, tumor cell susceptibility or resistance to NK lysis was a relatively unimportant determinant of liver-colonizing potential.

Original languageEnglish (US)
Pages (from-to)141-150
Number of pages10
JournalClinical & Experimental Metastasis
Volume13
Issue number2
DOIs
StatePublished - Mar 1 1995
Externally publishedYes

Keywords

  • colorectal neoplasms
  • gangliosides
  • metastasis
  • natural killer cells
  • sialytransferases

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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