Determination of celecoxib in human plasma using solid-phase extraction and high-performance liquid chromatography

H. H.Sherry Chow, Nathan Anavy, Dawn Salazar, Denise H. Frank, David S. Alberts

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


A simple reversed phase high-performance liquid chromatography (HPLC) method was developed for determination of celecoxib levels in human plasma. The procedure involves solid-phase extraction of celecoxib and the internal standard (SC-236) from plasma using C18 extraction cartridges. The chromatographic separation of celecoxib and SC-236 was achieved with a Nova Pak C8 column (3.8mm x 150mm) eluted with a mobile phase consisting of acetonitrile-tetrahydrofuran-sodium acetate buffer (pH 5.0) in the ratio of 30:8:62. An ultraviolet light detector with the wavelength set at 215nm was employed for detection. Celecoxib was well resolved from the plasma constituents and the internal standard. The extraction recovery of celecoxib and SC-236 from human plasma was greater than 88%. Linear calibration curves were established over a concentration range of 40-4000ng/ml when 0.25ml aliquots of plasma were used. The inter- and intra-day R.S.D. for the assay was less than 12 and 5%, respectively. This assay has been applied to the analysis of celecoxib levels in plasma samples collected from healthy participants entered into a Phase II clinical study.

Original languageEnglish (US)
Pages (from-to)167-174
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Issue number1
StatePublished - Jan 27 2004


  • Celecoxib
  • High-performance liquid chromatography
  • Human plasma

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry


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