Determination of d-limonene in adipose tissue by gas chromatography-mass spectrometry

Jessica A. Miller, Iman A. Hakim, Cynthia Thomson, Patricia Thompson, H. H. Sherry Chow

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We developed a novel method for analyzing d-limonene levels in adipose tissue. Fat samples were subjected to saponification followed by solvent extraction. d-Limonene in the sample extract was analyzed using gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring. Linear calibration curves were established over the mass range of 79.0-2529 ng d-limonene per 0.1 g of adipose tissue. Satisfactory within-day precision (R.S.D. 6.7-9.6%) and accuracy (%difference of -2.7 to 3.8%) and between-day precision (R.S.D. 6.0-10.7%) and accuracy (%difference of 1.8-2.6%) were achieved. The assay was successfully applied to human fat biopsy samples from a d-limonene feeding trial.

Original languageEnglish (US)
Pages (from-to)68-73
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume870
Issue number1
DOIs
StatePublished - Jul 1 2008

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Keywords

  • Adipose
  • GC-MS
  • d-Limonene

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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