Determination of joint loads using new sensate scaffolds for regenerating large cartilage defects in the knee

John Szivek, John T Ruth, Greg J. Heden, Michael A. Martinez, Nicklaus H. Diggins, Karl H. Wenger

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Two complete unicondylar surface replacement scaffold designs to support tissue-engineered cartilage growth that utilized adult endogenous stem cells were 3D printed and tested in a dog stifle model. Integrated rosette strain gauges were calibrated and used to determine shear loading within stifle joints for up to 12 months. An activity index that compared extent of daily activity with tissue formation showed differences in the extent and quality of new tissue with the most active animal having the most new tissue formation. Shear loads were highest early and decreased with time indicating that cartilage tissue formation begins while tissues experience high shear loads and continues as the loads decrease toward normal physiological levels. Scaffolds with biomimetic support pegs facilitated the most rapid bone ingrowth and were noted to have more cartilage formation with better quality cartilage as measured using both indentation testing and histology. Comparison of implant placement depth to previous studies suggested that placement depth affects the amount of tissue formation. This study provides measurements of loading patterns and cartilage regeneration on a complete medial condylar surface replacement that can be used for preclinical testing of a tissue engineering approach for the most common form of early stage osteoarthritis, unicondylar disease.

Original languageEnglish (US)
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
DOIs
StateAccepted/In press - 2016

Fingerprint

Cartilage
Scaffolds
Tissue
Defects
Histology
Biomimetics
Testing
Scaffolds (biology)
Strain gages
Stem cells
Tissue engineering
Indentation
Bone
Animals

Keywords

  • in vivo
  • Orthopedic
  • Regenerative medicine
  • Stem/progenitor cells
  • Tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Determination of joint loads using new sensate scaffolds for regenerating large cartilage defects in the knee. / Szivek, John; Ruth, John T; Heden, Greg J.; Martinez, Michael A.; Diggins, Nicklaus H.; Wenger, Karl H.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, 2016.

Research output: Contribution to journalArticle

@article{d524808ba9a14048b506a382a0b7a34d,
title = "Determination of joint loads using new sensate scaffolds for regenerating large cartilage defects in the knee",
abstract = "Two complete unicondylar surface replacement scaffold designs to support tissue-engineered cartilage growth that utilized adult endogenous stem cells were 3D printed and tested in a dog stifle model. Integrated rosette strain gauges were calibrated and used to determine shear loading within stifle joints for up to 12 months. An activity index that compared extent of daily activity with tissue formation showed differences in the extent and quality of new tissue with the most active animal having the most new tissue formation. Shear loads were highest early and decreased with time indicating that cartilage tissue formation begins while tissues experience high shear loads and continues as the loads decrease toward normal physiological levels. Scaffolds with biomimetic support pegs facilitated the most rapid bone ingrowth and were noted to have more cartilage formation with better quality cartilage as measured using both indentation testing and histology. Comparison of implant placement depth to previous studies suggested that placement depth affects the amount of tissue formation. This study provides measurements of loading patterns and cartilage regeneration on a complete medial condylar surface replacement that can be used for preclinical testing of a tissue engineering approach for the most common form of early stage osteoarthritis, unicondylar disease.",
keywords = "in vivo, Orthopedic, Regenerative medicine, Stem/progenitor cells, Tissue engineering",
author = "John Szivek and Ruth, {John T} and Heden, {Greg J.} and Martinez, {Michael A.} and Diggins, {Nicklaus H.} and Wenger, {Karl H.}",
year = "2016",
doi = "10.1002/jbm.b.33677",
language = "English (US)",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "0021-9304",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Determination of joint loads using new sensate scaffolds for regenerating large cartilage defects in the knee

AU - Szivek, John

AU - Ruth, John T

AU - Heden, Greg J.

AU - Martinez, Michael A.

AU - Diggins, Nicklaus H.

AU - Wenger, Karl H.

PY - 2016

Y1 - 2016

N2 - Two complete unicondylar surface replacement scaffold designs to support tissue-engineered cartilage growth that utilized adult endogenous stem cells were 3D printed and tested in a dog stifle model. Integrated rosette strain gauges were calibrated and used to determine shear loading within stifle joints for up to 12 months. An activity index that compared extent of daily activity with tissue formation showed differences in the extent and quality of new tissue with the most active animal having the most new tissue formation. Shear loads were highest early and decreased with time indicating that cartilage tissue formation begins while tissues experience high shear loads and continues as the loads decrease toward normal physiological levels. Scaffolds with biomimetic support pegs facilitated the most rapid bone ingrowth and were noted to have more cartilage formation with better quality cartilage as measured using both indentation testing and histology. Comparison of implant placement depth to previous studies suggested that placement depth affects the amount of tissue formation. This study provides measurements of loading patterns and cartilage regeneration on a complete medial condylar surface replacement that can be used for preclinical testing of a tissue engineering approach for the most common form of early stage osteoarthritis, unicondylar disease.

AB - Two complete unicondylar surface replacement scaffold designs to support tissue-engineered cartilage growth that utilized adult endogenous stem cells were 3D printed and tested in a dog stifle model. Integrated rosette strain gauges were calibrated and used to determine shear loading within stifle joints for up to 12 months. An activity index that compared extent of daily activity with tissue formation showed differences in the extent and quality of new tissue with the most active animal having the most new tissue formation. Shear loads were highest early and decreased with time indicating that cartilage tissue formation begins while tissues experience high shear loads and continues as the loads decrease toward normal physiological levels. Scaffolds with biomimetic support pegs facilitated the most rapid bone ingrowth and were noted to have more cartilage formation with better quality cartilage as measured using both indentation testing and histology. Comparison of implant placement depth to previous studies suggested that placement depth affects the amount of tissue formation. This study provides measurements of loading patterns and cartilage regeneration on a complete medial condylar surface replacement that can be used for preclinical testing of a tissue engineering approach for the most common form of early stage osteoarthritis, unicondylar disease.

KW - in vivo

KW - Orthopedic

KW - Regenerative medicine

KW - Stem/progenitor cells

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84963594099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84963594099&partnerID=8YFLogxK

U2 - 10.1002/jbm.b.33677

DO - 10.1002/jbm.b.33677

M3 - Article

C2 - 27080933

AN - SCOPUS:84963594099

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 0021-9304

ER -