Quantitative analysis of 100-µ nucleotide samples is accurately performed by analyzing total phosphorus concentration using an inductively coupled plasma (ICP) system. A single calibration using Na2HPO4(aq) is useful for all nucleotides. A detection limit of 750 ng of phosphorus Is determined with a relative standard deviation of 4.5 %. High-performance liquid chromatography (HPI.C) is used to separate mixtures of nucleotides on an anion exchange column using acetate buffers. The ICP Is used as a selective detector by observing P I emissions at 213.6 nm. A Babington nebulizer which Is used to aspirate the high percentage salt solutions with 6% efficiency Is described.
ASJC Scopus subject areas
- Analytical Chemistry