Determination of Nucleotides by Liquid Chromatography with a Phosphorus-Sensitive Inductively Coupled Plasma Detector

D. R. Heine, M. B. Denton, T. D. Schlabach

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Quantitative analysis of 100-µ nucleotide samples is accurately performed by analyzing total phosphorus concentration using an inductively coupled plasma (ICP) system. A single calibration using Na2HPO4(aq) is useful for all nucleotides. A detection limit of 750 ng of phosphorus Is determined with a relative standard deviation of 4.5 %. High-performance liquid chromatography (HPI.C) is used to separate mixtures of nucleotides on an anion exchange column using acetate buffers. The ICP Is used as a selective detector by observing P I emissions at 213.6 nm. A Babington nebulizer which Is used to aspirate the high percentage salt solutions with 6% efficiency Is described.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalAnalytical chemistry
Volume54
Issue number1
DOIs
StatePublished - Jan 1982

ASJC Scopus subject areas

  • Analytical Chemistry

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