Determination of nucleotides by liquid chromatography with a phosphorus-sensitive inductively coupled plasma detector

D. R. Heine, M Bonner Denton, T. D. Schlabach

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Quantitative analysis of 100-μL nucleotide samples is accurately performed by analyzing total phosphorus concentration using an inductively coupled plasma (ICP) system. A single calibration using Na2HP04(aq) is useful for all nucleotides. A detection limit of 750 ng of phosphorus is determined with a relative standard deviation of 4.5%. High-performance liquid chromatography (HPLC) is used to separate mixtures of nucleotides on an anton exchange column using acetate buffers. The ICP is used as a selective detector by observing P I emissions at 213.6 nm. A Babington nebulizer which is used to aspirate the high percentage salt solutions with 6% efficiency is described.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalAnalytical Chemistry
Volume54
Issue number1
StatePublished - 1982

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Liquid chromatography
Inductively coupled plasma
Phosphorus
Nucleotides
Detectors
High performance liquid chromatography
Buffers
Acetates
Salts
Calibration
Chemical analysis

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Determination of nucleotides by liquid chromatography with a phosphorus-sensitive inductively coupled plasma detector. / Heine, D. R.; Denton, M Bonner; Schlabach, T. D.

In: Analytical Chemistry, Vol. 54, No. 1, 1982, p. 81-84.

Research output: Contribution to journalArticle

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