Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp

Loc Tran, Linda Nunan, Rita M. Redman, Leone L. Mohney, Carlos R Pantoja-Morales, Kevin Fitzsimmons, Donald V Lightner

Research output: Contribution to journalArticle

265 Citations (Scopus)

Abstract

A new emerging disease in shrimp, first reported in 2009, was initially named early mortality syndrome (EMS). In 2011, a more descriptive name for the acute phase of the disease was proposed as acute hepatopancreatic necrosis syndrome (AHPNS). Affecting both Pacific white shrimp Penaeus vannamei and black tiger shrimp P. monodon, the disease has caused significant losses in Southeast Asian shrimp farms. AHPNS was first classified as idiopathic because no specific causative agent had been identified. However, in early 2013, the Aquaculture Pathology Laboratory at the University of Arizona was able to isolate the causative agent of AHPNS in pure culture. Immersion challenge tests were employed for infectivity studies, which induced 100% mortality with typical AHPNS pathology to experimental shrimp exposed to the pathogenic agent. Subsequent histological analyses showed that AHPNS lesions were experimentally in - duced in the laboratory and were identical to those found in AHPNS-infected shrimp samples collected from the endemic areas. Bacterial isolation from the experimentally infected shrimp enabled recovery of the same bacterial colony type found in field samples. In 3 separate immersion tests, using the recovered isolate from the AHPNS-positive shrimp, the same AHPNS pathology was reproduced in experimental shrimp with consistent results. Hence, AHPNS has a bacterial etiology and Koch's Postulates have been satisfied in laboratory challenge studies with the isolate, which has been identified as a member of the Vibrio harveyi clade, most closely related to V. parahemolyticus.

Original languageEnglish (US)
Pages (from-to)45-55
Number of pages11
JournalDiseases of Aquatic Organisms
Volume105
Issue number1
DOIs
StatePublished - Jul 9 2013

Fingerprint

Penaeidae
necrosis
shrimp
pathology
mortality
Vibrio harveyi
Vibrio parahaemolyticus
emerging diseases
Penaeus monodon
infectivity
etiology
Litopenaeus vannamei
lesion
aquaculture
pathogenicity
testing
farm
sampling
farms

Keywords

  • AHPNS
  • Early mortality syndrome
  • EMS
  • Etiology
  • Penaeus monodon
  • Penaeus vannamei
  • Vibrio

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. / Tran, Loc; Nunan, Linda; Redman, Rita M.; Mohney, Leone L.; Pantoja-Morales, Carlos R; Fitzsimmons, Kevin; Lightner, Donald V.

In: Diseases of Aquatic Organisms, Vol. 105, No. 1, 09.07.2013, p. 45-55.

Research output: Contribution to journalArticle

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