Detoxification and partial mineralization of the azo dye mordant orange 1 in a continuous upflow anaerobic sludge-blanket reactor

B. Donlon, E. Razo-Flores, M. Luijten, H. Swarts, G. Lettinga, James A Field

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

In batch toxicity assays, azo dye compounds were found to be many times more toxic than their cleavage products (aromatic amines) towards methanogenic activity in anaerobic granular sludge. Considering the ability of anaerobic microorganisms to reduce azo groups, detoxification of azo compounds towards methanogens can be expected to occur during anaerobic wastewater treatment. In order to test this hypothesis, the anaerobic degradation of one azo dye compound Mordant orange 1 (MO1), by granular sludge was investigated in three separate continuous upflow anaerobic sludge-blanket reactors. One reactor, receiving no cosubstrate, failed after 50 days presumably because of a lack of reducing equivalents. However, the two reactors receiving either glucose or a volatile fatty acids (acetate, propionate, butyrate) mixture, could eliminate the dye during operation for 217 days. The azo dye was reductively cleaved to less toxic aromatic amines (1,4-phenylenediamine and 5-aminosalicylic acid) making the treatment of MO1 feasible at influent concentrations that were over 25 times higher than their 50% inhibitory concentrations. In the reactor receiving glucose as cosubstrate, 5-aminosalicylic acid could only be detected at trace levels in the effluent after day 189 of operation. Batch biodegradability assays with the sludge sampled from this reactor confirmed the mineralization of 5-aminosalicylic acid to methane.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume47
Issue number1
DOIs
StatePublished - 1997
Externally publishedYes

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Azo Compounds
Detoxification
Azo dyes
Sewage
Mesalamine
Glucose
Acids
Poisons
Amines
Assays
Methanogens
Volatile fatty acids
Biodegradability
Wastewater treatment
Microorganisms
Toxicity
Volatile Fatty Acids
Butyrates
Effluents
Methane

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Detoxification and partial mineralization of the azo dye mordant orange 1 in a continuous upflow anaerobic sludge-blanket reactor. / Donlon, B.; Razo-Flores, E.; Luijten, M.; Swarts, H.; Lettinga, G.; Field, James A.

In: Applied Microbiology and Biotechnology, Vol. 47, No. 1, 1997, p. 83-90.

Research output: Contribution to journalArticle

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