Developing a photoautotrophic micropropagation system for woody plants

Toyoki Kozai, Chieri Kubota

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Our recent research has revealed that most chlorophyllous explants/plants in vitro including cotyledonary stage somatic embryos have the ability to grow photoautotrophically (without sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is due not to poor photosynthetic ability, but to the low CO2 concentration in the air-tight culture vessel during the photoperiod. Furthermore, we have shown that the photoautotrophic growth of several woody plants in vitro can be significantly promoted by increasing the CO2 concentration and light intensity in the vessel, by decreasing the relative humidity in the vessel, and by using a fibrous or porous supporting material with high air porosity instead of gelling agents such as agar. In this paper, the advantages of photoautotrophic micropropagation in a conventional, small culture vessel with a microporous gas filter for enhancing natural ventilation and in a large culture vessel with a forced ventilation unit are described for woody plants such as acacia (Acacia mangium), coffee (Coffea arabusta), eucalyptus (Eucalyptus camaldlensis), mangosteen (Garcinia mangostana), neem (Azadirachta indica), paulownia (Paulownia fortunei), and pine (Pinus radiata).

Original languageEnglish (US)
Pages (from-to)525-537
Number of pages13
JournalJournal of Plant Research
Volume114
Issue number1116
StatePublished - Dec 2001
Externally publishedYes

Fingerprint

Azadirachta indica
micropropagation
woody plants
Paulownia fortunei
carbon dioxide
Paulownia
Garcinia mangostana
mangosteens
Coffea
natural ventilation
gelling agents
Acacia mangium
air
Pinus radiata
Acacia
somatic embryos
Eucalyptus
porosity
light intensity
relative humidity

Keywords

  • Acacia mangium
  • Azadirachta indica
  • Coffea arabusta
  • Micropropagation
  • Photoautotrophy
  • Photosynthesis

ASJC Scopus subject areas

  • Plant Science

Cite this

Developing a photoautotrophic micropropagation system for woody plants. / Kozai, Toyoki; Kubota, Chieri.

In: Journal of Plant Research, Vol. 114, No. 1116, 12.2001, p. 525-537.

Research output: Contribution to journalArticle

@article{edbda98c9a5a4cee81eca8534e200c7b,
title = "Developing a photoautotrophic micropropagation system for woody plants",
abstract = "Our recent research has revealed that most chlorophyllous explants/plants in vitro including cotyledonary stage somatic embryos have the ability to grow photoautotrophically (without sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is due not to poor photosynthetic ability, but to the low CO2 concentration in the air-tight culture vessel during the photoperiod. Furthermore, we have shown that the photoautotrophic growth of several woody plants in vitro can be significantly promoted by increasing the CO2 concentration and light intensity in the vessel, by decreasing the relative humidity in the vessel, and by using a fibrous or porous supporting material with high air porosity instead of gelling agents such as agar. In this paper, the advantages of photoautotrophic micropropagation in a conventional, small culture vessel with a microporous gas filter for enhancing natural ventilation and in a large culture vessel with a forced ventilation unit are described for woody plants such as acacia (Acacia mangium), coffee (Coffea arabusta), eucalyptus (Eucalyptus camaldlensis), mangosteen (Garcinia mangostana), neem (Azadirachta indica), paulownia (Paulownia fortunei), and pine (Pinus radiata).",
keywords = "Acacia mangium, Azadirachta indica, Coffea arabusta, Micropropagation, Photoautotrophy, Photosynthesis",
author = "Toyoki Kozai and Chieri Kubota",
year = "2001",
month = "12",
language = "English (US)",
volume = "114",
pages = "525--537",
journal = "Journal of Plant Research",
issn = "0918-9440",
publisher = "Springer Japan",
number = "1116",

}

TY - JOUR

T1 - Developing a photoautotrophic micropropagation system for woody plants

AU - Kozai, Toyoki

AU - Kubota, Chieri

PY - 2001/12

Y1 - 2001/12

N2 - Our recent research has revealed that most chlorophyllous explants/plants in vitro including cotyledonary stage somatic embryos have the ability to grow photoautotrophically (without sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is due not to poor photosynthetic ability, but to the low CO2 concentration in the air-tight culture vessel during the photoperiod. Furthermore, we have shown that the photoautotrophic growth of several woody plants in vitro can be significantly promoted by increasing the CO2 concentration and light intensity in the vessel, by decreasing the relative humidity in the vessel, and by using a fibrous or porous supporting material with high air porosity instead of gelling agents such as agar. In this paper, the advantages of photoautotrophic micropropagation in a conventional, small culture vessel with a microporous gas filter for enhancing natural ventilation and in a large culture vessel with a forced ventilation unit are described for woody plants such as acacia (Acacia mangium), coffee (Coffea arabusta), eucalyptus (Eucalyptus camaldlensis), mangosteen (Garcinia mangostana), neem (Azadirachta indica), paulownia (Paulownia fortunei), and pine (Pinus radiata).

AB - Our recent research has revealed that most chlorophyllous explants/plants in vitro including cotyledonary stage somatic embryos have the ability to grow photoautotrophically (without sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is due not to poor photosynthetic ability, but to the low CO2 concentration in the air-tight culture vessel during the photoperiod. Furthermore, we have shown that the photoautotrophic growth of several woody plants in vitro can be significantly promoted by increasing the CO2 concentration and light intensity in the vessel, by decreasing the relative humidity in the vessel, and by using a fibrous or porous supporting material with high air porosity instead of gelling agents such as agar. In this paper, the advantages of photoautotrophic micropropagation in a conventional, small culture vessel with a microporous gas filter for enhancing natural ventilation and in a large culture vessel with a forced ventilation unit are described for woody plants such as acacia (Acacia mangium), coffee (Coffea arabusta), eucalyptus (Eucalyptus camaldlensis), mangosteen (Garcinia mangostana), neem (Azadirachta indica), paulownia (Paulownia fortunei), and pine (Pinus radiata).

KW - Acacia mangium

KW - Azadirachta indica

KW - Coffea arabusta

KW - Micropropagation

KW - Photoautotrophy

KW - Photosynthesis

UR - http://www.scopus.com/inward/record.url?scp=0012747304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0012747304&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0012747304

VL - 114

SP - 525

EP - 537

JO - Journal of Plant Research

JF - Journal of Plant Research

SN - 0918-9440

IS - 1116

ER -