Development and characterization of a monoclonal antibody against Taura syndrome virus

I. Côté, B. T. Poulos, R. M. Redman, D. V. Lightner

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We produced a panel of monoclonal antibodies (MAbs) from the fusion of Taura syndrome virus variants from Belize (TSV-BZ) immunized BALB/cJ mouse spleen cells and non-immunoglobulin secreting SP2/0 mouse myeloma cells. One antibody, 2C4, showed strong specificity and sensitivity for TSV in dot-blot immunoassay and immunohistochemistry (IHC) analysis. The MAb reacted against native TSV-BZ, TSV variants from Sinaloa, Mexico (TSV-SI) and TSV variants from Hawaii (TSV-HI) in dot-blot immunoassay. By IHC, the antibody identified the virus in a pattern similar to the digoxigenin-labelled TSV-cDNA probe for the TSV-BZ, TSV-HI and TSV-SI variants, but not for the TSV variants from Venezuela (TSV-VE) and the TSV variants from Thailand (TSV-TH). MAb 2C4 did not react against other shrimp pathogens or with normal shrimp tissue. Western blot analysis showed a strong reaction against CP2, a region of high antigenic variability amongst TSV variants. This antibody has potential diagnostic application in detection and differentiation of certain TSV biotypes.

Original languageEnglish (US)
Pages (from-to)989-996
Number of pages8
JournalJournal of Fish Diseases
Volume32
Issue number12
DOIs
StatePublished - Dec 1 2009

Keywords

  • Immunodiagnosis
  • Monoclonal antibody
  • Taura syndrome virus

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary (miscalleneous)

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