Development and characterization of a voltammetric carbon-fiber microelectrode pH sensor

Monique A. Makos, Donna M. Omiatek, Andrew G. Ewing, Michael L Heien

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

This work describes the development and characterization of a modified carbon-fiber microelectrode sensor capable of measuring real-time physiological pH changes in biological microenvironments. The reagentless sensor was fabricated under ambient conditions from voltammetric reduction of the diazonium salt Fast Blue RR onto a carbon-fiber surface in aprotic media. Fast-scan cyclic voltammetry was used to probe redox activity of the p-quinone moiety of the surface-bound molecule as a function of pH. In vitro calibration of the sensor in solutions ranging from pH 6.5 to 8.0 resulted in a pH-dependent anodic peak potential response. Flow-injection analysis was used to characterize the modified microelectrode, revealing sensitivity to acidic and basic changes discernible to 0.005 pH units. Furthermore, the modified electrode was used to measure dynamic in vivo pH changes evoked during neurotransmitter release in the central nervous system of the microanalytical model organism Drosophila melanogaster.

Original languageEnglish (US)
Pages (from-to)10386-10391
Number of pages6
JournalLangmuir
Volume26
Issue number12
DOIs
StatePublished - Jun 15 2010
Externally publishedYes

Fingerprint

pH sensors
Microelectrodes
carbon fibers
Carbon fibers
sensors
Sensors
Neurology
Cyclic voltammetry
Neurotransmitter Agents
Calibration
Salts
Flow Injection Analysis
neurotransmitters
Drosophila
central nervous system
Electrodes
Molecules
quinones
Drosophila melanogaster
organisms

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy
  • Medicine(all)

Cite this

Development and characterization of a voltammetric carbon-fiber microelectrode pH sensor. / Makos, Monique A.; Omiatek, Donna M.; Ewing, Andrew G.; Heien, Michael L.

In: Langmuir, Vol. 26, No. 12, 15.06.2010, p. 10386-10391.

Research output: Contribution to journalArticle

Makos, Monique A. ; Omiatek, Donna M. ; Ewing, Andrew G. ; Heien, Michael L. / Development and characterization of a voltammetric carbon-fiber microelectrode pH sensor. In: Langmuir. 2010 ; Vol. 26, No. 12. pp. 10386-10391.
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