Development of a bioartificial pancreas: II. Effects of oxygen on long- term entrapped βTC3 cell cultures

K. K. Papas, R. C. Long, A. Sambanis, I. Constantinidis

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

The effects of 3- and 4-day-long hypoxic episodes on the metabolic and secretory activities and on the levels of intracellular metabolites detectable by phosphorus-31 nuclear magnetic resonance of alginate/poly-L-lysine/alginate entrapped βCT3 mouse insulinomas continuously perfused with culture medium were studied. Results show that upon decreasing the oxygen concentration in the surrounding medium, the encapsulated cell system reached a new, lower metabolic and secretory state. Upon restoration of the oxygen concentration in the perfusion medium, all parameters returned to their prehypoxic levels within 2 to 3 days.

Original languageEnglish (US)
Pages (from-to)231-237
Number of pages7
JournalBiotechnology and Bioengineering
Volume66
Issue number4
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Keywords

  • Bioartificial pancreas
  • Bioenergetics
  • Cell encapsulation
  • Insulin secretion
  • Nuclear magnetic resonance (NMR) spectroscopy
  • Oxygen effects
  • βTC3 cells

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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