Development of a Fiber Optic Probe to Measure NIR Raman Spectra of Cervical Tissue in Vivo

Anita Mahadevan-Jansen, Michele Follen Mitchell, Nirmala Ramanujam, Urs Utzinger, Rebecca Richards-Kortum

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

The goal of this study was to develop a compact fiber optic probe to measure near infrared Raman spectra of human cervical tissue in vivo for the clinical diagnosis of cervical precancers. A Raman spectrometer and fiber optic probe were designed, constructed and tested. The probe was first tested using standards with known Raman spectra, and then the probe was used to acquire Raman spectra from normal and precancerous cervical tissue in vivo. Raman spectra of cervical tissue could be acquired in vivo in 90 s using incident powers comparable to the threshold limit values for laser exposure of the skin. Although some silica signal obscured tissue Raman bands below 900 cm -1, Raman features from cervical tissue could clearly be discerned with an acceptable signal-to-noise ratio above 900 cm -1. The success of the Raman probe described here indicates that near infrared Raman spectra can be measured in vivo from cervical tissues. Increasing the power of the excitation source could reduce the integration time to below 20 s.

Original languageEnglish (US)
Pages (from-to)427-431
Number of pages5
JournalPhotochemistry and Photobiology
Volume68
Issue number3
StatePublished - Sep 1998
Externally publishedYes

Fingerprint

Fiber optics
Raman scattering
fiber optics
Tissue
Raman spectra
probes
infrared spectra
Threshold Limit Values
Infrared radiation
Electric Power Supplies
Signal-To-Noise Ratio
Silicon Dioxide
Spectrometers
Signal to noise ratio
Skin
Lasers
signal to noise ratios
optics
spectrometers
silicon dioxide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Mahadevan-Jansen, A., Mitchell, M. F., Ramanujam, N., Utzinger, U., & Richards-Kortum, R. (1998). Development of a Fiber Optic Probe to Measure NIR Raman Spectra of Cervical Tissue in Vivo. Photochemistry and Photobiology, 68(3), 427-431.

Development of a Fiber Optic Probe to Measure NIR Raman Spectra of Cervical Tissue in Vivo. / Mahadevan-Jansen, Anita; Mitchell, Michele Follen; Ramanujam, Nirmala; Utzinger, Urs; Richards-Kortum, Rebecca.

In: Photochemistry and Photobiology, Vol. 68, No. 3, 09.1998, p. 427-431.

Research output: Contribution to journalArticle

Mahadevan-Jansen, A, Mitchell, MF, Ramanujam, N, Utzinger, U & Richards-Kortum, R 1998, 'Development of a Fiber Optic Probe to Measure NIR Raman Spectra of Cervical Tissue in Vivo', Photochemistry and Photobiology, vol. 68, no. 3, pp. 427-431.
Mahadevan-Jansen A, Mitchell MF, Ramanujam N, Utzinger U, Richards-Kortum R. Development of a Fiber Optic Probe to Measure NIR Raman Spectra of Cervical Tissue in Vivo. Photochemistry and Photobiology. 1998 Sep;68(3):427-431.
Mahadevan-Jansen, Anita ; Mitchell, Michele Follen ; Ramanujam, Nirmala ; Utzinger, Urs ; Richards-Kortum, Rebecca. / Development of a Fiber Optic Probe to Measure NIR Raman Spectra of Cervical Tissue in Vivo. In: Photochemistry and Photobiology. 1998 ; Vol. 68, No. 3. pp. 427-431.
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