Development of a Penaeus monodon‐type baculovirus (MBV) DNA probe by polymerase chain reaction and sequence analysis

C. C. LU, Feng-Jyu Tang-Nelson, G. H. KOU, S. N. CHEN

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Abstract. Penaeus monodon‐type baculovirus (MBV) was isolated and purified from the hepatopancreases of MBV‐infected Penaeus monodon Fabricius. MBV DNA was extracted and used as a template in a polymerase chain reaction (PCR). The primers were chosen from conserved regions of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus (AcNPV). One DNA fragment (674 base pairs) was amplified after PCR. There was a 65% homology between the predicted amino acid sequence of this PCR product with that of the polyhedrin polypeptide of AcNPV. Nucleotide sequence analysis indicated that the amplified DNA is the open reading frame of the MBV polyhedrin gene. This 674 bp DNA fragment was subsequently used as a probe in a dot blot analysis. The probe was able to hybridize with the DNA extracted from the purified MBV and from the MBV‐infected P. monodon, but not from the MBV uninfected P. monodon.

Original languageEnglish (US)
Pages (from-to)551-559
Number of pages9
JournalJournal of Fish Diseases
Volume16
Issue number6
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Penaeus
Penaeidae
Baculoviridae
DNA probes
DNA Probes
DNA-Directed DNA Polymerase
polymerase chain reaction
Sequence Analysis
sequence analysis
probe
Penaeus monodon
DNA
Polymerase Chain Reaction
Autographa californica multiple nucleopolyhedrovirus
Nucleopolyhedrovirus
virus
Hepatopancreas
gene
hepatopancreas
homology

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary (miscalleneous)

Cite this

Development of a Penaeus monodon‐type baculovirus (MBV) DNA probe by polymerase chain reaction and sequence analysis. / LU, C. C.; Tang-Nelson, Feng-Jyu; KOU, G. H.; CHEN, S. N.

In: Journal of Fish Diseases, Vol. 16, No. 6, 01.01.1993, p. 551-559.

Research output: Contribution to journalArticle

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AB - Abstract. Penaeus monodon‐type baculovirus (MBV) was isolated and purified from the hepatopancreases of MBV‐infected Penaeus monodon Fabricius. MBV DNA was extracted and used as a template in a polymerase chain reaction (PCR). The primers were chosen from conserved regions of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus (AcNPV). One DNA fragment (674 base pairs) was amplified after PCR. There was a 65% homology between the predicted amino acid sequence of this PCR product with that of the polyhedrin polypeptide of AcNPV. Nucleotide sequence analysis indicated that the amplified DNA is the open reading frame of the MBV polyhedrin gene. This 674 bp DNA fragment was subsequently used as a probe in a dot blot analysis. The probe was able to hybridize with the DNA extracted from the purified MBV and from the MBV‐infected P. monodon, but not from the MBV uninfected P. monodon.

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