Development of a sensitive assay for the measurement of antibodies against heat shock protein binding protein 1 (HspBP1): Increased levels of anti-HspBP1 IgG are prevalent in HIV infected subjects

Diana Papp, Zoltán Prohászka, Judit Kocsis, George Füst, Dénes Bánhegyi, Deborah A. Raynes, Vincent Guerriero

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The 70 kDa heat shock protein (Hsp70) is generally considered to be an intracellular protein, however, there is evidence that Hsp70 can be found in the extracellular environment. Hsp70 and antibodies against Hsp70 have been reported in human serum. Recent evidence has shown that Hsp70 antibodies are elevated in HIV infected individuals. This study reports on the antibody levels against a co-chaperone, HspBP1, that regulates Hsp70 activity. We have developed a solid-phase enzyme linked assay for the determination of anti-HspBP1 IgG antibodies. We report here that HspBP1 antibodies are present in human serum and the levels are elevated approximately twofold in HIV infected patients. There was no correlation between HspBP1 antibody levels and clinical parameters nor was there a relation between anti-Hsp70 levels and anti-HspBP1 levels. The presence of HspBP1 antibodies in human serum suggests that the protein may also be present in the serum. The increased level of HspBP1 antibodies in HIV infected individuals suggests a relationship directly to the virus or indirectly to secondary consequences of HIV infection.

Original languageEnglish (US)
Pages (from-to)464-469
Number of pages6
JournalJournal of Medical Virology
Volume76
Issue number4
DOIs
StatePublished - Aug 2005

Fingerprint

Heat-Shock Proteins
Protein Binding
Carrier Proteins
Immunoglobulin G
HIV
Antibodies
Serum
HIV Antibodies
HSP70 Heat-Shock Proteins
Enzyme Assays
HIV Infections
Blood Proteins
Viruses
Proteins

Keywords

  • Binding protein
  • Co-chaperone
  • Hsp70
  • Serum
  • Viral infection

ASJC Scopus subject areas

  • Virology

Cite this

Development of a sensitive assay for the measurement of antibodies against heat shock protein binding protein 1 (HspBP1) : Increased levels of anti-HspBP1 IgG are prevalent in HIV infected subjects. / Papp, Diana; Prohászka, Zoltán; Kocsis, Judit; Füst, George; Bánhegyi, Dénes; Raynes, Deborah A.; Guerriero, Vincent.

In: Journal of Medical Virology, Vol. 76, No. 4, 08.2005, p. 464-469.

Research output: Contribution to journalArticle

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