Development of alterations in hamster distal lung following exposure to fly ash from fluidized bed coal combustion: A morphometric study

Robert Clark Lantz, David E. Hinton

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Fly ash (30 mg) from an experimental fluidized bed coal combustor (FBC) was intratracheally instilled once into male Syrian golden hamsters. At 1, 3, 6, 9, or 30 days postadministration, lungs were fixed by intratracheal perfusion and tissues were processed for light and electron microscopic evaluation. Standard point count morphometry, used to analyze distal lung structures, revealed: an increase in volume density (Vv) of noncellular interstitial space at Day 1 which remained elevated at Day 3 but returned to control values subsequently; increased Vv for polymorphonuclear leukocytes (PMNs) and alveolar macrophages in alveolar air space at Day 6; increased Vv of cellular interstitium at Day 9; and increased Vv of noncellular interstitium at Day 30 following exposure. These observations are consistent with the hypothesis that the primary response of the distal lung to particle exposure is recruitment of PMNs and macrophages. When activated, these cells may release substances affecting the cellular and noncellular composition of the interstitial space, leading eventually to increased connective tissue in the interstitium.

Original languageEnglish (US)
Pages (from-to)132-139
Number of pages8
JournalToxicology and Applied Pharmacology
Volume82
Issue number1
DOIs
StatePublished - 1986
Externally publishedYes

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Coal Ash
Fluidized bed combustion
Coal
Coal combustion
Cricetinae
Tissue
Lung
Macrophages
Combustors
Fluidized beds
Mesocricetus
Alveolar Macrophages
Connective Tissue
Electrons
Neutrophils
Perfusion
Air
Chemical analysis
Light

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

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title = "Development of alterations in hamster distal lung following exposure to fly ash from fluidized bed coal combustion: A morphometric study",
abstract = "Fly ash (30 mg) from an experimental fluidized bed coal combustor (FBC) was intratracheally instilled once into male Syrian golden hamsters. At 1, 3, 6, 9, or 30 days postadministration, lungs were fixed by intratracheal perfusion and tissues were processed for light and electron microscopic evaluation. Standard point count morphometry, used to analyze distal lung structures, revealed: an increase in volume density (Vv) of noncellular interstitial space at Day 1 which remained elevated at Day 3 but returned to control values subsequently; increased Vv for polymorphonuclear leukocytes (PMNs) and alveolar macrophages in alveolar air space at Day 6; increased Vv of cellular interstitium at Day 9; and increased Vv of noncellular interstitium at Day 30 following exposure. These observations are consistent with the hypothesis that the primary response of the distal lung to particle exposure is recruitment of PMNs and macrophages. When activated, these cells may release substances affecting the cellular and noncellular composition of the interstitial space, leading eventually to increased connective tissue in the interstitium.",
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T1 - Development of alterations in hamster distal lung following exposure to fly ash from fluidized bed coal combustion

T2 - A morphometric study

AU - Lantz, Robert Clark

AU - Hinton, David E.

PY - 1986

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N2 - Fly ash (30 mg) from an experimental fluidized bed coal combustor (FBC) was intratracheally instilled once into male Syrian golden hamsters. At 1, 3, 6, 9, or 30 days postadministration, lungs were fixed by intratracheal perfusion and tissues were processed for light and electron microscopic evaluation. Standard point count morphometry, used to analyze distal lung structures, revealed: an increase in volume density (Vv) of noncellular interstitial space at Day 1 which remained elevated at Day 3 but returned to control values subsequently; increased Vv for polymorphonuclear leukocytes (PMNs) and alveolar macrophages in alveolar air space at Day 6; increased Vv of cellular interstitium at Day 9; and increased Vv of noncellular interstitium at Day 30 following exposure. These observations are consistent with the hypothesis that the primary response of the distal lung to particle exposure is recruitment of PMNs and macrophages. When activated, these cells may release substances affecting the cellular and noncellular composition of the interstitial space, leading eventually to increased connective tissue in the interstitium.

AB - Fly ash (30 mg) from an experimental fluidized bed coal combustor (FBC) was intratracheally instilled once into male Syrian golden hamsters. At 1, 3, 6, 9, or 30 days postadministration, lungs were fixed by intratracheal perfusion and tissues were processed for light and electron microscopic evaluation. Standard point count morphometry, used to analyze distal lung structures, revealed: an increase in volume density (Vv) of noncellular interstitial space at Day 1 which remained elevated at Day 3 but returned to control values subsequently; increased Vv for polymorphonuclear leukocytes (PMNs) and alveolar macrophages in alveolar air space at Day 6; increased Vv of cellular interstitium at Day 9; and increased Vv of noncellular interstitium at Day 30 following exposure. These observations are consistent with the hypothesis that the primary response of the distal lung to particle exposure is recruitment of PMNs and macrophages. When activated, these cells may release substances affecting the cellular and noncellular composition of the interstitial space, leading eventually to increased connective tissue in the interstitium.

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