Development of an antibody to actinomycin D and its application for the detection of serum levels by radioimmunoassay

A. R. Brothman, Thomas P Davis, J. J. Duffy, T. J. Lindell

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An antibody specific for actinomycin D (Act D) has been developed and used in a rapid, sensitive radioimmunoassay for detection of this anticancer drug in serum. The 2-amino group of the heterocyclic chromophore of Act D was covalently coupled to available free carboxyl groups of bovine serum albumin with carbodiimide. The resulting complex was then used for the production of a specific antibody to Act D in two male New Zealand rabbits. Antibody production was of sufficient titer in both rabbits to allow the development of a radioimmunoassay for the free drug which is rapid and sensitive enough to accurately measure 0.1 pmol of Act D. The antibody produced was characterized to be immunoglobulin G by virtue of its ability to bind to Protein A:Sepharose columns. With the use of Act-D analog, actinomine, the antibody was characterized to be specific for the pentapeptide portion of the molecule. Pharmacokinetic analysis of serial serum samples obtained from a patient who received the drug i.v. revealed a biphasic response with an α-serum half-life of 1.78 min and a β serum half-life of 34 min. An i.v. injection of Act D into a dog and assay of serum concentration revealed a similar biphasic response with an α serum half-life of 0.78 min and a β-serum half-life of of 208 min.

Original languageEnglish (US)
Pages (from-to)1184-1187
Number of pages4
JournalCancer Research
Volume42
Issue number3
StatePublished - 1982

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Dactinomycin
Radioimmunoassay
Antibodies
Half-Life
Serum
Pharmaceutical Preparations
Rabbits
Carbodiimides
Bovine Serum Albumin
Antibody Formation
Pharmacokinetics
Immunoglobulin G
Dogs
Injections

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Development of an antibody to actinomycin D and its application for the detection of serum levels by radioimmunoassay. / Brothman, A. R.; Davis, Thomas P; Duffy, J. J.; Lindell, T. J.

In: Cancer Research, Vol. 42, No. 3, 1982, p. 1184-1187.

Research output: Contribution to journalArticle

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