Abstract
An antibody specific for actinomycin D (Act D) has been developed and used in a rapid, sensitive radioimmunoassay for detection of this anticancer drug in serum. The 2-amino group of the heterocyclic chromophore of Act D was covalently coupled to available free carboxyl groups of bovine serum albumin with carbodiimide. The resulting complex was then used for the production of a specific antibody to Act D in two male New Zealand rabbits. Antibody production was of sufficient titer in both rabbits to allow the development of a radioimmunoassay for the free drug which is rapid and sensitive enough to accurately measure 0.1 pmol of Act D. The antibody produced was characterized to be immunoglobulin G by virtue of its ability to bind to Protein A:Sepharose columns. With the use of Act-D analog, actinomine, the antibody was characterized to be specific for the pentapeptide portion of the molecule. Pharmacokinetic analysis of serial serum samples obtained from a patient who received the drug i.v. revealed a biphasic response with an α-serum half-life of 1.78 min and a β serum half-life of 34 min. An i.v. injection of Act D into a dog and assay of serum concentration revealed a similar biphasic response with an α serum half-life of 0.78 min and a β-serum half-life of of 208 min.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1184-1187 |
| Number of pages | 4 |
| Journal | Cancer Research |
| Volume | 42 |
| Issue number | 3 |
| State | Published - 1982 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Development of an antibody to actinomycin D and its application for the detection of serum levels by radioimmunoassay. / Brothman, A. R.; Davis, Thomas P; Duffy, J. J.; Lindell, T. J.
In: Cancer Research, Vol. 42, No. 3, 1982, p. 1184-1187.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Development of an antibody to actinomycin D and its application for the detection of serum levels by radioimmunoassay
AU - Brothman, A. R.
AU - Davis, Thomas P
AU - Duffy, J. J.
AU - Lindell, T. J.
PY - 1982
Y1 - 1982
N2 - An antibody specific for actinomycin D (Act D) has been developed and used in a rapid, sensitive radioimmunoassay for detection of this anticancer drug in serum. The 2-amino group of the heterocyclic chromophore of Act D was covalently coupled to available free carboxyl groups of bovine serum albumin with carbodiimide. The resulting complex was then used for the production of a specific antibody to Act D in two male New Zealand rabbits. Antibody production was of sufficient titer in both rabbits to allow the development of a radioimmunoassay for the free drug which is rapid and sensitive enough to accurately measure 0.1 pmol of Act D. The antibody produced was characterized to be immunoglobulin G by virtue of its ability to bind to Protein A:Sepharose columns. With the use of Act-D analog, actinomine, the antibody was characterized to be specific for the pentapeptide portion of the molecule. Pharmacokinetic analysis of serial serum samples obtained from a patient who received the drug i.v. revealed a biphasic response with an α-serum half-life of 1.78 min and a β serum half-life of 34 min. An i.v. injection of Act D into a dog and assay of serum concentration revealed a similar biphasic response with an α serum half-life of 0.78 min and a β-serum half-life of of 208 min.
AB - An antibody specific for actinomycin D (Act D) has been developed and used in a rapid, sensitive radioimmunoassay for detection of this anticancer drug in serum. The 2-amino group of the heterocyclic chromophore of Act D was covalently coupled to available free carboxyl groups of bovine serum albumin with carbodiimide. The resulting complex was then used for the production of a specific antibody to Act D in two male New Zealand rabbits. Antibody production was of sufficient titer in both rabbits to allow the development of a radioimmunoassay for the free drug which is rapid and sensitive enough to accurately measure 0.1 pmol of Act D. The antibody produced was characterized to be immunoglobulin G by virtue of its ability to bind to Protein A:Sepharose columns. With the use of Act-D analog, actinomine, the antibody was characterized to be specific for the pentapeptide portion of the molecule. Pharmacokinetic analysis of serial serum samples obtained from a patient who received the drug i.v. revealed a biphasic response with an α-serum half-life of 1.78 min and a β serum half-life of 34 min. An i.v. injection of Act D into a dog and assay of serum concentration revealed a similar biphasic response with an α serum half-life of 0.78 min and a β-serum half-life of of 208 min.
UR - http://www.scopus.com/inward/record.url?scp=0020029193&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020029193&partnerID=8YFLogxK
M3 - Article
C2 - 6174214
AN - SCOPUS:0020029193
VL - 42
SP - 1184
EP - 1187
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0099-7013
IS - 3
ER -