Development of an in vitro primary cell culture system from the penaeid shrimp, Penaeus stylirostris and Penaeus vannamei

RenéA Luedeman, Donald V Lightner

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Studies were carried out to improve the methods for producing primary cell cultures of penaeid shrimp and to make these procedures routine and practical. Supplements tested included: lobster hemolymph, shrimp muscle extract, fetal bovine serum and hybridoma quality fetal bovine serum. Grace's Insect Medium supplemented with hybridoma quality fetal bovine serum was found to provide the best results. The optimum conditions for shrimp cells in Grace's Insect Medium were determined to be an osmolality of approximately 700-750 mmol/kg, a temperature range of 25-28 °C and incubation in a normal atmosphere. Using the methods developed, monolayers of primary cultures of ovarian epithelioid cells from Penaeus stylirostris and Penaeus vannamei were routinely obtained with 80% confluence within a 2-day period.

Original languageEnglish (US)
Pages (from-to)205-211
Number of pages7
JournalAquaculture
Volume101
Issue number3-4
DOIs
StatePublished - Feb 15 1992

Fingerprint

Litopenaeus stylirostris
Penaeidae
fetal bovine serum
Litopenaeus vannamei
serum
cell culture
hybridomas
shrimp
insect
insects
confluence
osmolality
lobster
lobsters
hemolymph
muscle
incubation
cells
muscles
atmosphere

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Development of an in vitro primary cell culture system from the penaeid shrimp, Penaeus stylirostris and Penaeus vannamei. / Luedeman, RenéA; Lightner, Donald V.

In: Aquaculture, Vol. 101, No. 3-4, 15.02.1992, p. 205-211.

Research output: Contribution to journalArticle

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