Development of an integrated fingerstick blood self-collection device for radiation countermeasures

Jian Gu, Alan Norquist, Carla Brooks, Mikhail Repin, Sanjay Mukherjee, Jerome Lacombe, Jianing Yang, David J. Brenner, Sally Amundson, Frederic Zenhausern

Research output: Contribution to journalArticle

Abstract

We report the development of system for packaging critical components of the traditional collection kit to make an integrated fingerstick blood collector for self-collecting blood samples of 100 μl or more for radiation countermeasures. A miniaturized vacuum tube system (VacuStor system) has been developed to facilitate liquid reagent storage, simple operation and reduced sample contamination. Vacuum shelf life of the VacuStor tube has been analyzed by the ideal gas law and gas permeation theory, and multiple ways to extend vacuum shelf life beyond one year have been demonstrated, including low temperature storage, Parylene barrier coating and container vacuum bag sealing. Self-collection was also demonstrated by healthy donors without any previous fingerstick collection experience. The collected blood samples showed similar behavior in terms of gene expression and cytogenetic biodosimetry assays comparing to the traditionally collected samples. The integrated collector may alleviate the sample collection bottleneck for radiation countermeasures following a large-scale nuclear event, and may be useful in other applications with its self-collection and liquid reagent sample preprocessing capabilities.

Original languageEnglish (US)
Article numbere0222951
JournalPloS one
Volume14
Issue number10
DOIs
StatePublished - Jan 1 2019

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Vacuum
Blood
Radiation
Equipment and Supplies
blood
Gases
Electron tubes
Liquids
collectors
Gene expression
Permeation
sampling
Containers
Assays
Packaging
shelf life
Product Packaging
Contamination
Cytogenetics
gases

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Development of an integrated fingerstick blood self-collection device for radiation countermeasures. / Gu, Jian; Norquist, Alan; Brooks, Carla; Repin, Mikhail; Mukherjee, Sanjay; Lacombe, Jerome; Yang, Jianing; Brenner, David J.; Amundson, Sally; Zenhausern, Frederic.

In: PloS one, Vol. 14, No. 10, e0222951, 01.01.2019.

Research output: Contribution to journalArticle

Gu, J, Norquist, A, Brooks, C, Repin, M, Mukherjee, S, Lacombe, J, Yang, J, Brenner, DJ, Amundson, S & Zenhausern, F 2019, 'Development of an integrated fingerstick blood self-collection device for radiation countermeasures', PloS one, vol. 14, no. 10, e0222951. https://doi.org/10.1371/journal.pone.0222951
Gu, Jian ; Norquist, Alan ; Brooks, Carla ; Repin, Mikhail ; Mukherjee, Sanjay ; Lacombe, Jerome ; Yang, Jianing ; Brenner, David J. ; Amundson, Sally ; Zenhausern, Frederic. / Development of an integrated fingerstick blood self-collection device for radiation countermeasures. In: PloS one. 2019 ; Vol. 14, No. 10.
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