Development of high-density DNA microarray membrane for profiling smoke- and hydrogen peroxide-induced genes in a human bronchial epithelial cell line.

K. Yoneda, K. Peck, M. M. Chang, K. Chmiel, Y. P. Sher, J. Chen, P. C. Yang, Yin Chen, R. Wu

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Development of the high-density DNA microarray technique permits the analysis of thousands of genes simultaneously for their differential expression patterns in various biological processes. Through clustering analysis and pattern recognition, the significance of differentially expressed genes can be recognized and correlated with biological events that may take place inside the cell and tissue. With this notion in mind, high-density DNA microarray nylon membrane with colorimetry detection was used to profile the expression of smoke- and hydrogen peroxide-inducible genes in a human bronchial epithelial cell line, HBE1. On the basis of the time course of expression, at least three phases of change in gene expression could be recognized. The first phase is an immediate event in response to oxidant injury. This phase includes induction of the bcl-2 and mdm-2 genes, which are involved in the regulation of apoptosis, and the mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) gene, that functions as a regulator of various mitogen-activated protein kinase activities. The second phase, usually 5 h later, includes the induction of various stress proteins and ubiquitin, which are important in providing the chaperone mechanism and the turnover of damaged macromolecules. The third phase, which is 5-10 h later, includes the induction of genes that are apparently involved in reducing oxidative stress by metabolizing reactive oxygen species. In this phase, enzymes associated with tissue and cell remodeling are also elevated. These results demonstrate a complex gene expression array by bronchial epithelial cells in response to the insult of oxidants that are relevant to environmental pollutants.

Original languageEnglish (US)
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume164
Issue number10 Pt 2
StatePublished - Nov 15 2001
Externally publishedYes

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Oligonucleotide Array Sequence Analysis
Smoke
Hydrogen Peroxide
Epithelial Cells
Cell Line
Membranes
Genes
Oxidants
Mitogen-Activated Protein Kinase Phosphatases
Dual Specificity Phosphatase 1
Colorimetry
Biological Phenomena
Gene Expression
Environmental Pollutants
Nylons
Ubiquitin
Heat-Shock Proteins
Mitogen-Activated Protein Kinases
Cluster Analysis
Reactive Oxygen Species

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Development of high-density DNA microarray membrane for profiling smoke- and hydrogen peroxide-induced genes in a human bronchial epithelial cell line. / Yoneda, K.; Peck, K.; Chang, M. M.; Chmiel, K.; Sher, Y. P.; Chen, J.; Yang, P. C.; Chen, Yin; Wu, R.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 164, No. 10 Pt 2, 15.11.2001.

Research output: Contribution to journalArticle

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