Development of presynaptic inhibition onto retinal bipolar cell axon terminals is subclass-specific

Timm Schubert, Daniel Kerschensteiner, Erika D Eggers, Thomas Misgeld, Martin Kerschensteiner, Jeff W. Lichtman, Peter D. Lukasiewicz, Rachel O L Wong

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Synaptic integration is modulated by inhibition onto the dendrites of postsynaptic cells. However, presynaptic inhibition at axonal terminals also plays a critical role in the regulation of neurotransmission. In contrast to the development of inhibitory synapses onto dendrites, GABAergic/glycinergic synaptogenesis onto axon terminals has not been widely studied. Because retinal bipolar cells receive subclass-specific patterns of GABAergic and glycinergic presynaptic inhibition, they are a good model for studying the development of inhibition at axon terminals. Here, using whole cell recording methods and transgenic mice in which subclasses of retinal bipolar cells are labeled, we determined the temporal sequence and patterning of functional GABAergic and glycinergic input onto the major subclasses of bipolar cells. We found that the maturation of GABAergic and glycinergic synapses onto the axons of rod bipolar cells (RBCs), ON-cone bipolar cells (ON-CBCs) and OFF-cone bipolar cells (OFF-CBCs) were temporally distinct: spontaneous chloride-mediated currents are present in RBCs earlier in development compared with ON- and OFF-CBC, and RBCs receive GABAergic and glycinergic input simultaneously, whereas in OFF-CBCs, glycinergic transmission emerges before GABAergic transmission. Because ON-CBCs show little inhibitory activity, GABAergic and glycinergic events could not be pharmacologically distinguished for these bipolar cells. The balance of GABAergic and glycinergic input that is unique to RBCs and OFF-CBCs is established shortly after the onset of synapse formation and precedes visual experience. Our data suggest that presynaptic modulation of glutamate transmission from bipolar cells matures rapidly and is differentially coordinated for GABAergic and glycinergic synapses onto distinct bipolar cell subclasses.

Original languageEnglish (US)
Pages (from-to)304-316
Number of pages13
JournalJournal of Neurophysiology
Volume100
Issue number1
DOIs
StatePublished - Jul 2008
Externally publishedYes

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Retinal Bipolar Cells
Presynaptic Terminals
Synapses
Dendrites

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Schubert, T., Kerschensteiner, D., Eggers, E. D., Misgeld, T., Kerschensteiner, M., Lichtman, J. W., ... Wong, R. O. L. (2008). Development of presynaptic inhibition onto retinal bipolar cell axon terminals is subclass-specific. Journal of Neurophysiology, 100(1), 304-316. https://doi.org/10.1152/jn.90202.2008

Development of presynaptic inhibition onto retinal bipolar cell axon terminals is subclass-specific. / Schubert, Timm; Kerschensteiner, Daniel; Eggers, Erika D; Misgeld, Thomas; Kerschensteiner, Martin; Lichtman, Jeff W.; Lukasiewicz, Peter D.; Wong, Rachel O L.

In: Journal of Neurophysiology, Vol. 100, No. 1, 07.2008, p. 304-316.

Research output: Contribution to journalArticle

Schubert, T, Kerschensteiner, D, Eggers, ED, Misgeld, T, Kerschensteiner, M, Lichtman, JW, Lukasiewicz, PD & Wong, ROL 2008, 'Development of presynaptic inhibition onto retinal bipolar cell axon terminals is subclass-specific', Journal of Neurophysiology, vol. 100, no. 1, pp. 304-316. https://doi.org/10.1152/jn.90202.2008
Schubert, Timm ; Kerschensteiner, Daniel ; Eggers, Erika D ; Misgeld, Thomas ; Kerschensteiner, Martin ; Lichtman, Jeff W. ; Lukasiewicz, Peter D. ; Wong, Rachel O L. / Development of presynaptic inhibition onto retinal bipolar cell axon terminals is subclass-specific. In: Journal of Neurophysiology. 2008 ; Vol. 100, No. 1. pp. 304-316.
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